LOCUS       sequence-121846-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in 
            minTol backbone; also with cry:cfp cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(97..120)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(295..314)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     564..582
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(716..736)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(761..790)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(824..853)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(863..892)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(902..931)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggagctgcc gctgccggtg ctgccggtgc catgccccag gaacaggtgg tggcggccct
       61 cggagcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
      121 tggtgtccac gtagtagtag ccgggcagtt gcacgggctt cttggccatg tagatggtct
      181 tgaactccac caggtagtgg ctgccgtcct tcagcttcag ggcctggtgg atctcgccct
      241 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct
      301 tctgcattac ggggccgtcg ggggggaagt tggtgccgcg catcttcacc ttgtagatca
      361 gcgtgccgtc ctgcagggag gagtcctggg tcacggtcac cagaccgccg tcctcgaagt
      421 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtaa tcggggatgt
      481 cggcggggtg cttcacgtac gccttggagc cgtacatgaa ctggggggac aggatgtccc
      541 aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
      601 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg gagccctcca
      661 tgcgcacctt gaagcgcatg aactctttga tgacctcctc gcccttgctc accatcacct
      721 tcctcttctt cttgggcatg gaattcgaga tctctaaatt caagtcctct tcagaaatga
      781 gcttttgctc catggtgagg tcgcccaagc tctccatttc attcaagtcc tcttcagaaa
      841 tgagcttttg ctccatttca ttcaagtcct cttcagaaat gagcttttgc tccatttcat
      901 tcaagtcctc ttcagaaatg agcttttgct cca
//
