LOCUS       sequence-121864-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into
            a MIN-tagged locus using Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Dnmt3b1-Poly(A)-PuroR
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    126..163
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     polyA_signal    192..416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     405..422
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
       61 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      121 cacggatgat cctgacgacg gagaccgccg tcgtcgacaa gccgggatgc tggttctttc
      181 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
      241 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
      301 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
      361 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
      421 ggctgatcag cgagctctag atcatcgata ccgcgacact ggccgctcta gagctcagtt
      481 agcctccccc atctcccggg caaacgtgcg cgccaggtcg catatcgtcg gtatggagcc
      541 gggggtggtg acgtgggtct ggaccatccc ggaggtaagt tgcagcaggg cgtcccggca
      601 gccggcgggc gattggtcgt aatccaggat aaagacgtgc atggaacgga ggcgtttggc
      661 caagacgtcc aaggcccagg caaacacgtt atacaggtcg ccgttggggg ccagcaactc
      721 gggggcccga aacagggtaa ataacgtgtc cccgatatgg ggtcgtgggc ccgcgt
//
