LOCUS       sequence-121869-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into
            a MIN-tagged locus using Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Dnmt3b1-Poly(A)-PuroR
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      306..315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             395..406
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
       61 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      121 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      181 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      241 gcatggacga gctgtacaag agcggcctga ggagcagagc ccaggcgagc aacagcgccg
      301 tggacgccac catgggcgat cgcatgaagg gagacagcag acatctgaat gaagaagagg
      361 gtgccagcgg gtatgaggag tgcattatcg ttaatgggaa cttcagtgac cagtcctcag
      421 acacgaagga tgctccctca cccccagtct tggaggcaat ctgcacagag ccagtctgca
      481 caccagagac cagaggccgc aggtcaagct cccggctgtc taagagggag gtctccagcc
      541 ttctgaatta cacgcaggac atgacaggag atggagacag agatgatgaa gtagatgatg
      601 ggaatggctc tgatattcta atgccaaagc tcacccgtga gaccaaggac accaggacgc
      661 gctctgaaag cccggctgtc cgaacccgac atagcaatgg gacctccagc ttggagaggc
      721 aaagagcctc ccccagaatc acccgaggtc ggcagggccg ccaccatgtg caggagtacc
      781 ctgtggagtt tccggctacc aggtctcgga gacgtcgagc atcgtcttca gcaagcacgc
      841 catggtcatc ccctgccagc gtcgacttca tggaagaagt gacacctaag agcgtcagta
      901 ccccatcagt tgacttgagc caggatggag atcaggaggg tatggatacc acacaggtgg
      961 atgcagagag cagagatgga gacagcacag agt
//
