LOCUS       sequence-121907-        3118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  qacR 2 downstream of a Tet promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   de Los Santos EL, Meyerowitz JT, Mayo SL, Murray RM
  TITLE     Engineering Transcriptional Regulator Effector Specificity Using 
            Computational Design and In Vitro Rapid Prototyping: Developing a 
            Vanillin Sensor.
  JOURNAL   ACS Synth Biol. 2015 Aug 19.
  PUBMED    26262913
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Aug 19."
COMMENT     Sequence Label: pES003 (pTet-qacR 2)
FEATURES             Location/Qualifiers
     source          1..3118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             123..782
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(189..208)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      803..897
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1321..1338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1455..2000)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1535..1554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2243..2261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2305..2316
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2897..2914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2930..3001
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3017..3044
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gctatgaagc tgagaccttg atcgggcacg taagaggttc caactttcac cataatgaaa
       61 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
      121 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
      181 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
      241 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
      301 ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg
      361 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
      421 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
      481 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
      541 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
      601 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
      661 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
      721 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
      781 aatttgatat cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc
      841 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
      901 agcctcggtg agaatccaag cctcgatcaa cgtctcattt tcgccaaaag ttggcccagg
      961 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     1021 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     1081 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     1141 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg gtagcggagt
     1201 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     1261 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     1321 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     1381 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     1441 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     1501 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     1561 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     1621 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     1681 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     1741 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     1801 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     1861 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     1921 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     1981 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     2041 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     2101 aattctcatg tttgacagct tatcatcgat aagcttccga tggcgcgccg agaggcttta
     2161 cactttatgc ttccggctgg tctcagcatc cacgcattat aacgtcgtat tagctgcctt
     2221 tcgtcttcaa taattcttga catccctatc agtgatagag atacttcgtg caatttttat
     2281 atcaccgcca ggggtacaac aagcaaagag gagaaatact agcatgaact tgaaagataa
     2341 gattttgggt gttgctaaag agctgtttat caaaaacggt tacaatgcta ctaccactgg
     2401 tgagattgtg aaattgtctg aatcttctaa agggaatcta tactatcatt tcaaaacgaa
     2461 ggaaaatttg ttcttggaaa tcttgaacat tcaactgagt aaatatcaag aacaatggaa
     2521 aaaggagcag attaaagcta aaactaatag agaaaagttt tatctgtata atgagttgtc
     2581 tttgactaca caatattact atccactaca gaacgcacag atcgagttct acactgaata
     2641 ctataagact aactccatca atgaaaaaca gaacaaatac caaaataagt atatcgatgc
     2701 atatcacgtt attttcaaag aaggtaatct taacggtgag tggagtataa atgatgttaa
     2761 tgccgtttct aaaattgctg ctatggctgt tttaggtatt gttatgttca cacatgagca
     2821 aaacatcaac gaaagaatta agttgatgaa caagttctct cagatctttt tgaatggtct
     2881 tagtaaaggt tctggtcacc atcatcacca ccattaatga accaggcatc aaataaaacg
     2941 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     3001 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata gtcgggcacg
     3061 actctcaagg gcatcggtcg acacagaaaa gcccgccttt cggcgggctt tgctcgag
//
