LOCUS       sequence-121920-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            mCherry 8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G06
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..238
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(285..590)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(387..406)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttattttat tttattttat tttattttat tttattttat tttattttat ttaaccatga
       61 cagtaatgta taaagtctgt aaagacatta aacacgtaag tgaaaccatg ggcacaagtt
      121 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      181 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggg
      241 gcccgtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      301 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      361 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
      421 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg
      481 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
      541 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
      601 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
      661 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
      721 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
      781 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
      841 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
      901 atctggcttt tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt
      961 actgttgtaa ttcattaagc attctgccga catgg
//
