LOCUS       sequence-121921-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            mCherry myc-tag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G08
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    104..228
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(275..580)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(377..396)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttattttat tttattttat tttattttat tttattttat ttaaccatga cagtaatgta
       61 taaagtctgt aaagacatta aacacgtaag tgaaaccatg ggcacaagtt tgtacaaaaa
      121 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      181 aaaacagact acataatact gtaaaacaca acatatccag tcactatggg gcccgtcgac
      241 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
      301 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
      361 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
      421 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
      481 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
      541 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg
      601 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
      661 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
      721 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
      781 tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg gtatacatat
      841 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcat
//
