LOCUS       sequence-121922-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term 
            sfGFP 8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G10
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..233
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(280..585)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(382..401)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tttattttat tttattttat tttattttat tttattttat tttatttaac catgacagta
       61 atgtataaag tctgtaaaga cattaaacac gtaagtgaaa ccatgggcac aagtttgtac
      121 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      181 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggggcccg
      241 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
      301 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
      361 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
      421 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
      481 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      541 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      601 tctcgtcagc aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct
      661 gattttccgc tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc
      721 ttaccagacc ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc
      781 aactgtcact gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac
      841 atatcagtat atattcttat accggcaaaa atcagcgcgc aaaatacgcc atactgttat
      901 ctgggctttt
//
