LOCUS       sequence-121923-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with C-term eGFP
            8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G04
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..197
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(244..549)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(346..365)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttattttatt taaccatgac agtaatgtat aaagtctgta aagacattaa acacgtaagt
       61 gaaaccatgg gcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
      121 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      181 catatccagt cactatgggg cccgtcgacc tgcagactgg ctgtgtataa gggagcctga
      241 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      301 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
      361 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc
      421 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      481 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
      541 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
      601 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgca
//
