LOCUS       sequence-121926-       19671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of luciferase operon in Tn7 insertion site of Gram 
            negative bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19671)
  AUTHORS   Howe K, Karsi A, Germon P, Wills RW, Lawrence ML, Bailey RH
  TITLE     Development of stable reporter system cloning luxCDABE genes into 
            chromosome of Salmonella enterica serotypes using Tn7 transposon.
  JOURNAL   BMC Microbiol. 2010 Jul 23;10:197. doi: 10.1186/1471-2180-10-197.
  PUBMED    20653968
REFERENCE   2  (bases 1 to 19671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2010-07-23"; volume: "10:197. doi"; pages: " 
            10.1186/1471-2180-10-197"
COMMENT     Sequence Label: pBEN276
FEATURES             Location/Qualifiers
     source          1..19671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6265..6281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6265..6281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6278..6300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6289..6305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6313..6319,6320..6337,6338..6343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6358..6379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     mobile_element  7230..7395
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(7828..8778)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8826..9048
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     9731..9750
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            9788..9897
                     /note="incP origin of transfer"
     primer_bind     9920..9939
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(10068..10859,10860..10928))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10691..10710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10929..11020)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(11039..11066)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(11158..11244)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     11397..11414
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(18105..18389)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(18163..18182)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             18416..19294
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcgaggaatt cttcccgtga tggataaata agggtaagat tccgcgtaag tatcgcgggg
       61 gcgtaagtct ggttataagg cgttattgtt gcaggcagtt tggtcacggc cagcgcgcag
      121 caaccggagc gtacaaaagt acgtgaggat ggcgagcact gcccggggcc aaaatggcaa
      181 ataaaatagc ctaataatcc agacgattac ccgtaatatg tttaatcagg gctatactta
      241 gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca aggattcgta
      301 acctcgaggt acaaccagtt tgcaagatga ttaaacaata acttaagttg aagttacccc
      361 attaaatgga tggctaatat gactaaaaaa atttcattca ttattaacgg ccaggttgaa
      421 atttttcccg aaagtgatga tttagtgcaa tccattaatt ttggtgataa tagtgtttac
      481 ctgccaatat tgaataattc tcatgtaaaa aacattattg attataatga aaataataaa
      541 ttacggttgc ataatattgt caattttctc tatacggtag ggcaaagatg gaaaaatgaa
      601 gaatattcaa gacgcaggac atacattcgt gatttaaaaa aatatatggg atattcagaa
      661 gcaatggcca agttagaggc caactggata tctatgattt tatgttctaa aggtggcctt
      721 tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg gctacctcag
      781 gatgaaagtt atattaaggc ttttccgaaa ggtaagtcta tacatctgtt ggcaggtaat
      841 gttccattat ctgtgatcat gtctatatta cgcgcaattt taaccaagaa tcagtgtatt
      901 ataaaaacat cgtcaaccga tccctttacc gctaatgcat tagcgttaag ctttatcgat
      961 gtagacccta atcatccgat aacgcgctct ttgtctgttg tatattggcc acaccaaggt
     1021 gatacatcac tcgcaaaaga aattatgcaa catatggatg ttattgtcgc ttggggaggg
     1081 gaagatgcga ttaattgggc tgtagaacat gcaccaccct atgctgacgt gattaaattt
     1141 ggctctaaaa agagtttttg cattattgat aatccagttg atttaacgtc agcagctacc
     1201 ggtgcggctc atgatatttg tttttacgat cagcgcgctt gtttttctgc ccaaaacata
     1261 tattacatgg gaaatcagta tgaggaattt aagttagcgt tgatagaaaa acttaatcta
     1321 tatgcgcata tattaccaaa cgccaaaaaa gattttgatg aaaaggcggc ctattcttta
     1381 gtccaaaaag agagcttatt tgctggatta aaagtagagg tggatgttca tcaacgttgg
     1441 atgattattg agtcaaatgc gggtgtggaa tttaatcaac cacttggcag atgtgtgtat
     1501 cttcatcacg tcgataatat tgagcaagta ttgccttatg ttcaaaaaaa taagacacaa
     1561 accatatcta tttttccttg ggaatccgca tttaagtatc gagatgcgtt ggcattaaga
     1621 ggtgcggaaa ggattgtaga agcaggaatg aataatatat ttcgagttgg tggatctcat
     1681 gacggaatga ggccgttaca acgattagtg acatatattt ctcatgagag gccatctcat
     1741 tatactgcta aggatgttgc ggttgaaata gaacagactc gattcctgga agaagataag
     1801 ttccttgtat ttgtcccgta ataggtaaaa agtatggaaa ataaatccaa atataaaacc
     1861 atcgaccatg ttctttgtgt tgaaggaaat aaaaaaattc atgtttggga aacgctgcca
     1921 gaagaaacca gcccaaagag aaagaatccc attattattg cgtcgggttt tgcccgaagg
     1981 atggatcatt ttgctggttt agcggaatat ttatcgcgga atgggtttca tgtgattcgc
     2041 tatgattcac ttcaccacgt tgggttgagt tcagggacaa ttgatgaatt tacaatgtct
     2101 ataggaaaac agagcctatt agccgtggtt gattggttaa atacacgaaa aataaataac
     2161 cgtggtattt tggcttcaag cttatctgca cggatagttt atgcaagtct atctgaaatt
     2221 aatgtttcat ttttaatcac cgcagtcggt gttgttaact taagatatac gcttgaaaga
     2281 gctttaggat ttgattatct cagtttaccc attaatgaat tgccgaataa tttggatttt
     2341 gaaggccata aattgggtgc tgaagtcttt gcgagagatt gccttgattt tggctgggaa
     2401 gatttaactt ctacaatcaa tagcatgatg tatcttgata taccgtttat tgcttttact
     2461 gcaaataacg acaattgggt aaagcaagat gaagttatca cattgttatc aaatattcgt
     2521 agtaatcgat gcaagataca ttctttgtta ggaagttcgc atgacttgtg tgttttctta
     2581 gtggtcctgc gcaattttta tcaatcggtt acgaaggctg ctatcgcgat ggataatgat
     2641 cgtctggata ttgatgttga tattattgaa ccatcattcg aacatctaac tattgcgaca
     2701 gtcaatgaac gtcgaatgaa aattgagatt gaaaatcaag cgatttcgct gtcttaaaac
     2761 ctattgggat agatattacc ctatagattt caagatggat cgcgacggca agggagcgaa
     2821 tcccgggagc atagcaaact atgtgaccgg ggtgagtgag tgcagccaac aaagaagcaa
     2881 cttgaaagat aacgggtata gttaattcta tcactcaaat ataagggctc tctatgaaat
     2941 ttggaaactt tttgcttaca taccaacccc cccaattttc tcaaacagaa gtaatgaaac
     3001 gtttggttaa attaggtcgt atttctgagg agtgtggttt tgatactgta tggttactgg
     3061 agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac
     3121 ttggtgcaac caaaaaattg aatgtaggga ctgcggctat tgttcttccc accgctcatc
     3181 cagtgcgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt
     3241 ttggtatttg tcgggggctt tacaataaag actttcgcgt atttggcacg gatatgaata
     3301 acagtcgcgc tttaacggag tgctggtacg ggttgataaa aaatggcatg acagagggat
     3361 atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac ccgacagcat
     3421 atagtaaagg tggagcccct gtttatgtgg ttgctgaatc agcctcgaca actgaatggg
     3481 ccgctcaatt tggtttaccg atgatattaa gttggattat aaatactaac gaaaagaaag
     3541 cacagcttga gctttataac gaggtggctc aagaatatgg gcacgatatt cataatatcg
     3601 accattgctt atcatatata acatctgtaa attatgactc aaataaagcg aaagagattt
     3661 gtcggaaatt tctagggcat tggtatgatt cttatgtgaa tgccacgacc atttttgatg
     3721 attcagacaa aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa
     3781 agggacatag agatactaat cgccgcattg attacagtta cgaaatcaat cccgtgggaa
     3841 ccccgcagga atgcattgac ataattcaaa aagacattga tgccacggga atatcaaata
     3901 tctgttgtgg gtttgaagcg aatggaacag tagacgaaat tattgcttcc atgaagctct
     3961 tccagtctga tgtcatgccg tttcttaaag aaaaacaacg ttcgctatta tagtagctaa
     4021 ggaaaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca
     4081 agaacaaagt atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga
     4141 acagattttg gtgtatgaaa atcatttttc aggtaatggt gttgtcggtg ctcctctgac
     4201 tgtttctggt tttttgctcg gtttaacaga aaaaattaaa attggctcat tgaatcacat
     4261 cattacaact catcatcctg tccgaatagc ggaggaggct tgcttactgg atcaattaag
     4321 cgaagggaga tttattttag ggtttagtga ttgtgaaaaa aaagatgaaa tgcgtctttt
     4381 taatcgccct gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga
     4441 tgctttaaca acaggctatt gtaatcccga taatgatttt tatagtttcc ctaaaatatc
     4501 ggtaaacccc cacgcttata cccaaggcgg gcctcggaga tatattacag caaccagtca
     4561 tcatattgtt gaatgggcgg ctaaaaaagg cattcctctc atctttaagt gggatgactc
     4621 caatgatgtt agatatgaat atgctgaaag gtataaagcc gttgctgata aatatggcat
     4681 tgacttatca gcgatagatc atcagttaat ggtattggtt aactataacg aagatagtca
     4741 caaagctaaa caagagacgc gtgcatttat ccgtgattat gttcttgaaa tgtatcctaa
     4801 tgaaaatctc gaaaataaac ttgaagagat aatcacagaa aacgctgtcg gagattatac
     4861 ggaatgtata gctgcggcta agctggcaat tgaaaagtgc ggtgcaaaaa gggtattatt
     4921 atcctttgaa ccaatgaatg acttgatgca ccaaaaaaat gtaatcaata ttgttgatga
     4981 taatattaaa aagtaccaca tgtagtaaaa gaatatggca gcaacgctgc catattctct
     5041 aaattatttg gaggggtaaa acaggtatga cttcatatgt tgataaacaa gagatcatag
     5101 caagctcaga aattgatgat ttgatttttt ccagcgatcc attagcttgg tcttacgatg
     5161 aacaggaaaa aatcagaaac aaatttgttc ttgatgcatt tcgtaatcac tataaacatt
     5221 gtcaagaata ccgtcactac tgtcaggtac acaaagtaga cgacaatatt acggaaattg
     5281 atgacatacc tgtattccca acatcagttt ttaagtttac tcgcttatta acttctcagg
     5341 agaacgagat tgaaagttgg tttaccagca gcggcacgag tggtttaaaa agtcaggtgg
     5401 cgcgtgacag actaagtatt gagagactct taggctctgt gagttatggc atgaaatatg
     5461 ttggtagttg gtttgatcat caaatagagt tggtcaactt agggccagat agatttaatg
     5521 ctcataacat ttggtttaaa tatgttatta gtttggtaga attattatat cccacgacat
     5581 ttaccgtaat ggaagaacga atagattttg ttaagacatt gaatagcctt gagcgaataa
     5641 aaaatcaagg gaaagatatt tgtcttatcg gctcaccata ctttatttat ttgctctgcc
     5701 agtatatgaa agataaaaac atctcatttt atggggataa aaacctttat atcataacgg
     5761 ggggcggctg gaaaagttat gaaaaagagt ccctaaaacg cgatgatttc aatcatcttt
     5821 tattcgacac gttcaacctc aataatatta gtcaaatccg cgatatattt aatcaagttg
     5881 aactcaacac ttgtttcttt gaggatgaaa tgcaacgtaa acgtgttccg ccgtgggtat
     5941 atgcgcgagc acttgatcct gaaacattga aacctgtacc tgatggaatg ccgggtttga
     6001 tgagttatat ggatgcgtca tcaacgagtt atccggcatt tattgttacc gatgatgtcg
     6061 ggataatgag cagagaatat ggtcaatatc ctggtgtact tgttgagatt ttacgtcgcg
     6121 tcaatacgag ggcacagaaa gggtgtgctt taagcttaaa ccaagcattt aatagttgat
     6181 atactctatg gattttaaga tgcatcgcga tggcaaggga gtgaattcgg atccgagctc
     6241 ggtaccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6421 gtcgtgccag gactacggtc gtgccaactt gcgtgataac tatcgtctgg ttgatggcgc
     6481 atcgaaagac ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa
     6541 caagtttgtt gttcagtacg ctactgactc gatgacctcg cagggtaaag ggctgtcgca
     6601 gggttctggc gttgcatttg ataacgaaaa atttgcctac aatatcaaca acaacggtca
     6661 catgctgcgt atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta
     6721 cgtgggtatg taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt
     6781 cggtattcgc ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta
     6841 cgacaacgtc gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc
     6901 acaacaatgg caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac
     6961 ctacgccaag tgggatgaga aatggggtta cgactacacc ggtaacgctg ataacaacgc
     7021 gaacttcggc aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag
     7081 cgacgagtgg accttcggtg cccagatgga aatctggtgg taagcttgcg cggccgctat
     7141 tgacccggga tctgattaat taaaacgtgg cttactagga tccgatatca tttaaatcta
     7201 gggataacag ggtaatacta gtgtcgacca accagataag tgaaatctag ttccaaacta
     7261 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
     7321 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
     7381 ttttgtccgc ccacaggctg aaagcgctat ttcttccaga attgccatga ttttttcccc
     7441 acgggaggcg tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc
     7501 tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt
     7561 tcatgttcta gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt
     7621 tcatctgtta cattgtcgat ctgttcatgg tgaacagctt taaatgcacc aaaaactcgt
     7681 aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt
     7741 ccctttgata tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca
     7801 ctgatagata caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc
     7861 tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact
     7921 ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc
     7981 atcgtgtagt gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg
     8041 tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg
     8101 tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac
     8161 caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt
     8221 aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag
     8281 gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc
     8341 ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat
     8401 gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt
     8461 ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg
     8521 attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata
     8581 agcattttcc ctactgatgt tcatcatctg aacgtattgg ttataagtga acgataccgt
     8641 ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat
     8701 tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa
     8761 aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt
     8821 gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa
     8881 attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc
     8941 tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa
     9001 agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc
     9061 agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc
     9121 ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc
     9181 ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg
     9241 cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc
     9301 atgcaaggaa actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta
     9361 tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg
     9421 attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca
     9481 cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtcggg gatcgacgct
     9541 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     9601 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     9661 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     9721 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     9781 tgccgccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     9841 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcgg
     9901 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     9961 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    10021 gcgcgtaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10381 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10681 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10981 agcggataca tatttgaatg tatttagaaa aataaacaaa agagtttgta gaaacgcaaa
    11041 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
    11101 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
    11161 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
    11221 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc
    11281 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
    11341 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
    11401 taatctgtat caggctgaaa atcttctctc atccgccaaa acagccaagc ttgcatgcct
    11461 gcaggtcgac tctagaggat cccctaaaaa taatgaccga gctagctcta actgtgggat
    11521 gtgataacga gtcttgctat atggtgtttc agcttcgtaa ataaatgaac tctgcgctcc
    11581 atccttgttg cgtacggaag caaaggacga gagattagga aaatcgatta ttttggctcg
    11641 ttgaagatcc gatgcttcaa actcaatcac acgatccgcc ttatttattt gctttgttgt
    11701 actgttaacc accttagctc gacttaataa aggcaaatga gtaaatcgag tccatttccg
    11761 ttcttgcatt ggattaaacc aaacaaaaat acgccactcc ttatgacccg agttacggaa
    11821 taaatgacca atatgtacaa cctgcacatt gtcattaaat gtagctagcc taaccactca
    11881 ctcttcccca taaaccattt ccaagaatct ttgtgcttcc tcagttatcc gctctttaga
    11941 taacgttgca cttcttaata atcgccagcg cctaagctca acatcttcct gtttaagctt
    12001 aataaaagct tggctaatcc gtctaatttg ataatcttcc acactctctg agtaacgctt
    12061 taagcaaagc gctaccaaag gcagtttctg tagatttttt gcaagagagg ttccgttagg
    12121 agtttgcttt aacagccagc tcgatgtcgc tcttgggtga tcaaggctac tatctagacg
    12181 ctttatgatt cttaatagtt gtcgtacagc aattcgatct ctttggttcc aatcaactct
    12241 aggggcgggt gccagacgct cttgttgatg ctgttgattc cagtgaacta gccaatccct
    12301 gtcatgtcgg taaagccaag cgtatagcac cccaccctct aaagactgtc ttgccgcctt
    12361 aattccttgg tatttatgca ctagttgctg ccagtcttta tgcttaacag ataaatcttc
    12421 agagttaggt tgcacagact ggctaacagg tcttgtcgtt atagagtgct cagtaagagc
    12481 acttgcctgc tgtagcgctt ctataaccgt tagttttggc aataaggctt gccacacaat
    12541 actatgctgt aaataactaa aggcttttct atgcttacgg aatatacttt tcagccaaca
    12601 cgtgtccttg ttctctgcca actttaaatc cagtttctct agtgcctcat cactaaaagt
    12661 ctgcctcact ctctccgcca ccaagtcatg acgaatgtgc ttgcttttgg ttagccctag
    12721 atcctgcgct aagcgctgat aaaacagcgt ccactgctca aggcttgggg aaagctcttg
    12781 cgctcgtgga gcatctaaca gaggggctat ataagcagct agtgctgtta attgagatag
    12841 ggagtctttg gggtagtctg aaagcagctc agtatgaccc aaagcccaaa attgatgtcg
    12901 gtgatcatct acagctctat caaagaagac taaagcaccg tgttttggac aatatggcaa
    12961 agcgggcaaa taccaatctc gttgccaaaa ggcttcccca tacctattta gctgaagagc
    13021 aacgcaatca gggcagtatc taaagcggtt atcgctctta actctagaag cagcgactcc
    13081 tagcattaaa tgcaccgcac cttgcgcttg gtactccatt aaccgaatag cttcgtctcg
    13141 gcgctccttg cctacaaacg gagcatataa agggaataag gtatgctcat aaataagctg
    13201 ctgaacagcg taacgtcctg tttgatgtag atgtcttgct atcacaccta aatgcgaggg
    13261 cagacctaag gtagcgacca ccttgcggtt gccatacacc tcatccaaca gctgcttagg
    13321 actaacaatc ccttgataaa cgcctgcccg tgcaatagtg ctataaatca gctcattcga
    13381 gtacggaaca ggaaagtttc tcatggctaa cctgcttgtt taaataagct cgccatatcc
    13441 actattaccc ctttcccttt taaccgttca tgcatggttt tttcaggttg gcgttgtgaa
    13501 tagatataac gtaaatccgt atcaggcaag ctatcccagt cgctgggctt gactaccttt
    13561 atagccgaaa cctttttact cttggagggc ttttctagtt ctgacactac cgtttcgcct
    13621 tccatcaacc actgcaaaac aagaggcagt aacttttgtc ttgtcatcgt tggattctgg
    13681 ctaaacgctt ttttaatagt gggaattaac aggcttgaat cgtaatcctc tttcagcatc
    13741 agatataaat gacgctgatc ttcggtatct aactcttgaa gggctttttc ttctggtgtt
    13801 tgttcttgta tcgctgcgat atctagctga agttggatta accgtttatc aatctcggga
    13861 acgactagat cagaataacg agcaatgcgt tctgggatac ccgagcgtaa tgcctctagc
    13921 atggggtgca caggctttaa ctcatcttga tacacttgcc gcaataaacc agcggtaata
    13981 cgctcattgc ctaaagctag cgcacggagc tgagcgagta caaaaagttt tactacaatg
    14041 tccatcactc cttggcttag ctcataccac acatcacgga cctcatccga taacagcgca
    14101 tctttgcgtt gtaaaagctg taattgccaa agattatccg taaaagcgat ccactcttga
    14161 ttgggctttc cacgttgcgt ttgttgtata ggatcccaga atatagctcc aaaccctgcc
    14221 cctctacgtg cagaccgcaa atcagcctca aaaatctctc gtgctttagg ggtaccaatc
    14281 aacatcactg gtacgccaat aatattcacc atcgtcacaa aaaagttcag catctcttga
    14341 gatccacccg aacgagagcg gcttaaatgc tgaatttcat caataaccaa caaccctaaa
    14401 gcatgtgcat tggctatttg cgacatcaaa gccaacatgg tttctatacc atgacgtttt
    14461 aagccataac gacgctcata gttcgagccc aaggctcgat ccaacgctct gaaaaaattc
    14521 aagcagattt cttttagcga accattatgc gagcagtcta ttttcaaata caccacctgc
    14581 tctacattga gttcacgatg gtaaatcacc tgaggatacg tggctagaat acgatgaaga
    14641 gaggtcgtct tcccactacc agaacaacca attaacaata agctttgtgc cgtagatcgt
    14701 gcctcctcaa agcgaaatgt ctccaactct cccgtttgaa cacgctcata accattttgt
    14761 aaatgctttt gtaaatctcc tgttttagga tttctgccta cgtagccacc tcgaatcatg
    14821 accgaaatac gctcacttag tagcaaatgc gtacctaatg gctgaaaata gtcatctgga
    14881 atacgacaaa tggtatgagc tctgataaca cgggactttt gcaagtcaga ggaagtaagc
    14941 tgtaaagagg atttcagtga tgcagcacta ttcactgact cttgtaatgg tggtaaggcc
    15001 tcgataaaag gattatcacg ataagcctct acccccgtat cacgataaac tgcttgaatc
    15061 cgggtagcac tcatgactca tccttttctg gtggatcctg aaataattca ggcacgtatg
    15121 tgggtaggct gtaatcttct tgatcatccg cttccactgc gttgaaagga ataactttag
    15181 cctcatcacc tgaagagctt ggcttcaaat gctccgcacg ttttttacgc tccgaggtca
    15241 cggcttcttt tttattagtt ttaatctgct taatgcgtgt tgatttgggc tcagtagtac
    15301 tgggcgttaa cttattcgct ttctgaatgg tttgctgaat aaacgcctca agctccctgc
    15361 gtttagttaa ctcatcctgc ttcgcattgg ctttattgtg tttttcttgt gcttgtatat
    15421 cccaaacctc ccaaaatgag agacctttaa actgccgact acgttccgtc agattacagc
    15481 gccaaaatac acggctgcca acttgcggaa acaaataaat cgtatcaacc agcactgggt
    15541 cataagccgc ttctaaatgt tgaggtctag ctatatcagt gctccgctgc aaccaaccct
    15601 cacgcagaat ctctgacccc gagtaataca aaccccacaa attaacgcca aatgaagaaa
    15661 tagagacctt tcggcgaggc agtaacgcta ctcgcaactg ctcttgctcc acagccctta
    15721 aactacctgt acgatgctgc ataccccatt gccatagctg gacaggaata gacggtaaat
    15781 ctgtaggaaa atcagcatct cgatcgtatt tatccatcac cagatggtta tttctgaata
    15841 agatcgtacg caaaataatt tgtgtgaact caaataccga cagagatgca tctaacctat
    15901 agtctgtttc accatggctt ttgatccgac tgccctctac aatgccaggt gcaaaggact
    15961 taaactcggc ttgtagtgtt ctaaaagtgc tttccactat gcctttagca tcgccacgtc
    16021 taggtggagc actttccact cgcacattaa aactagaaac taaggcttcg acctgatgac
    16081 tcattaattc gccacggtcc gctagcaaca catctggcaa acctacacac ggccagtctg
    16141 agctactaat ctcaatatca tgctgggcac aaatggccgt tttgtcagag caagcattta
    16201 caaaagcctg catcgccacc acataagacg gattttcaaa gccgatataa aagcccgtga
    16261 tcatccgact aaacacatca atcacaatgt aaagcgttgg tcttcctatg attttttggc
    16321 gatcatgatg atccactaaa taaatatcag caatcgtggc atcaatctca taacgactcc
    16381 cagggcctaa cgcctgagaa gtggctgtac tacttaaggg tcgtacgtct tttttatata
    16441 cccctgcttt aactctagac tttaagcgct gagctttagg gtattctcga tcataaaaat
    16501 aacgaaactg acgtagtgtt gggtaatcct cttggggaat gcgaggaaaa tactgagcaa
    16561 acaagtccac aaatcgtcta taggcaacgg tggtctttgt acctttttga tttaacaggt
    16621 gcttttctat ggtcaaccta aaaaggcgtt caatctcggg cgttaccttg gttccttcac
    16681 ccttaccata ttctctggct cggccaatct ttgctgttcc tgtcgctgaa cgtctttccc
    16741 ctggtgcacc gctgtttttg tagtcaggaa ttaatgcatt aggcgtttga ccacgctgcc
    16801 agtaacggcg taacaactta taaactgtag ccttagtaac cttatgttct tggaccacat
    16861 gctcaacgag ttcgcttctg actttagggt cgaaacgatc cttactatta ataataggaa
    16921 gaatttttcg ataatcctcc tcgcgcttct ggaagctgac agaatctaca gacggctctt
    16981 ctaaatcaag atgtacataa gggtcatcag ttctaactaa gcggccttca tctaaatact
    17041 gcattagcaa caactcagcc ctagcttgtg gaactccttt atcagcgctt atttgcatcc
    17101 agacaacttg gccatcctct atagccaaaa tgcgatacgg atcattatca aatagcacaa
    17161 cctcattaat ttgccacata gcgcaactcc tccatattca ctacttggct aatacagaga
    17221 tctgcacact tatttgccct gaaagactta taaatattga acttaataaa accatttgcg
    17281 gttaaggctc gtatctcact caatgttttg cctaactcca aatcataagc aatatcaacc
    17341 tgcttacaga cattgataat gttttcatct cctttttctt gcaggatatg ggccaatggg
    17401 gatagttgtg ctaaaagctc cgcagaaact tcttctgttt tcactgaata aagccattca
    17461 atattttctt ttactacggg atttatttct ttatcagtaa aaatgaacca aggaatttgc
    17521 ttttgctgcc aatagcgacg ctctagttct agtttttcta aggtacgctc gtcttgtaag
    17581 gctgctgcag gtttgacttg aatagcaaac tgctcaaaag gaccatcttt gcagtccact
    17641 aaaaaatcag tagacataac ctgatctaca ccacgaataa caggatgctt aataccacta
    17701 tctattgcaa tctgcctggt atcactaggt aataagggga actgctcgcg tatatctagc
    17761 acgctgctct cccactcaag actgagaaaa acagcaagct ctaagtcaga tagcaaatga
    17821 tggactcgtc ccgtcttatg agaataaata cggtgggaac gacctgaaga aggaacttct
    17881 tgtactgtta gccatggaat atagtcttta ccatgccctt ggccacgccc ctctttaata
    17941 cgacgggcaa tttgcacttc agaaaatgaa gagtttgctt tagccataac aaaagtccag
    18001 tatgcttttt cacagcataa ctggactgat ttcagtttac aactattctg tctagtttaa
    18061 gacttgggta ccgagctcga attcgctagc ccaaaaaaac gggtatggag aaacagtaga
    18121 gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa atgctatggc
    18181 atagcaaagt gtgacgccgt gcaaataatc aatgtggact tttctgccgt gattatagac
    18241 acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa tgcttttaat
    18301 aagcggggtt accggtttgg ttagcgagaa gagccagtaa aagacgcagt gacggcaatg
    18361 tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga aaagtatggc
    18421 tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aatgcccatc tggtggcggg
    18481 tttaacgccg attgaggcca acggttatct cgattttttt atcgaccgac cgctgggaat
    18541 gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa atcagggacg
    18601 agaatttgtt tgccgaccgg gtgatatttt gctgttcccg ccaggagaga ttcatcacta
    18661 cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg gtttactttc gtccgcgcgc
    18721 ctactggcat gaatggctta actggccgtc aatatttgcc aatacggggt tctttcgccc
    18781 ggatgaagcg caccagccgc atttcagcga cctgtttggg caaatcatta acgccgggca
    18841 aggggaaggg cgctattcgg agctgctggc gataaatctg cttgagcaat tgttactgcg
    18901 gcgcatggaa gcgattaacg agtcgctcca tccaccgatg gataatcggg tacgcgaggc
    18961 ttgtcagtac atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca
    19021 gcatgtttgc ttgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag
    19081 cgtcttaagc tggcgcgagg accaacgtat cagccaggcg aagctgcttt tgagcaccac
    19141 ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt gacgatcaac tctatttctc
    19201 gcgggtattt aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga
    19261 aaaagtgaat gatgtagccg tcaagttgtc ataattggta acgaatcaga caattgacgg
    19321 cttgacggag tagcataggg tttgcagaat ccctgcttcg tccatttgac aggcacatta
    19381 tgcatcgatg ataagctgtc aaacatgagc agatcctcta cgccggacgc atcgtggcct
    19441 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
    19501 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
    19561 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
    19621 tattaaagag gggcgtgggg tcgacgcggc cgtggcgcgc ctcctaggtg c
//
