LOCUS       sequence-121928-       10927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Read-out loxP mCherry to GFP switch for integration on CxTi10816, 
            Mos chr IV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR02
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2999..3023
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3030..3046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3030..3046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3985..4018)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4047..4067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5368..5401)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5430..5450
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5463..5765,5819..5893,5945..6064,6116..6331)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6563..6580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6563..6579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8520..8531
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8677..8701)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10136..10156)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10360..10379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10479..10501
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10539..10557)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10625..10729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10730..10798,10799..10927,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgc ggtacccaag gcgacaacgt aagtgactga aggcattaaa
     1621 ggtacatgcg tccgcgcaag tgtccgcttt tgctggccta acaagtaaga gaaacaagta
     1681 agaaagagta agagaaaact cacccgaggt gtctcttcta gcaaccgcct attcagataa
     1741 ttttaagcca gttcgagagt tatttgtcgc atggtataga agctcgttat ttacattaaa
     1801 tttttagtct tcccgcagaa gtggacgatg cgatgggcta cttcaaaaat ctgacgccac
     1861 gtgtcaagga ccttcacaag agcatgacaa acttggaaga tattagcaag tggcagaaga
     1921 aggccagttt ctctggcacc cttgagaatt actcgcgtct cggtgacaag atcaatgtga
     1981 aaccatttat ggatgctgtt gatgctagaa tgggtgccga agctgatgcc gtgaaagggg
     2041 tataaagcta ctgttgcacg acgactcaca gactaatcat tccaggtcct cgcgatttgt
     2101 gaaaaataca agtcattcta ccaaaacgag ggaaaacttc acgcggacag tatcgccaat
     2161 ttgaatagga ctcggctcga catggacagt gcggcggata aatatgcgaa caacgagact
     2221 gaagttaaca agactcgttt ggataacagt accacggaat ttgaagtggc ttgtgagagg
     2281 tttgtaggtc tttcgtaggc acgatatcct tatttgaatg tgtttcagaa tgcgagaact
     2341 ggcgaacgga atcaagacaa ttgaatcgaa ccattcttcc tggcaagacg gtcttatgaa
     2401 ggagattaaa gtggcgttgc gtaaataaag aaaggatcct ctttgaaaat aaagattttc
     2461 tacattcact tattttttta gctttatact tttcagatcg aatgaactgt ttccaacgtt
     2521 tccatattaa ttttttttag ttaaaaaagt tgggtattaa cagttttgtt aacagttttg
     2581 tcttccgaca gatgtgagac ggaaaaatgg ccaaaattcg agattttgac taaaatcagt
     2641 gtagcttttt caaaattttg aaccggcatg aaaaagtttg aaaagttttc gagaagtttc
     2701 aatccgttca tttgagccca tgttgggtct ataagatcaa aatcgcccga actgttagtc
     2761 ctcctttaag ttacgattat taacaaaatg tcgcctaagt ttagtttcac acttgtctca
     2821 cactagtcga tgaactgtgt gactactgta gtgagtacag tagggcccgt gtaaaaatac
     2881 aaaaaccaag aaaaagtaaa ccaaaataaa aaacgaattt attgttcact acaaccaaac
     2941 tgcaaagttc ttttcttacc catcagctca atcgtgtact tgcgtggaaa ccctaggagc
     3001 cactttgtac aagaaagctg ggtcacgggc aggaaacagc tatgaccatg attacgccaa
     3061 gctacgtaat acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc
     3121 tcgaggaatt cctgcagctg cagttcaatc ggtttttcct tgcttgcgcc caattctgat
     3181 ggttcttcca tttctaaatt ttagtttaat aaaaaaattt taaaataata cgagttttga
     3241 gactaacttt caataaaaac caaaaaaaat tgcttcaaaa aagagttcgc gccgatttac
     3301 gataaacaca gcgcgctacg acactccgaa aaccgcgccg caccattaca ttctccgccc
     3361 caccgcacgg tttgcggaag gcgatgaaat tgctgggcct aaaattttaa cccttttttt
     3421 cccgtttttc aactgttttc ccgatttttt cgatattttt ttcaggtttt tagttagaaa
     3481 agtatataaa aattgcatta tttgtagttt tcgctcgtaa tttgtgatag aaaacggcca
     3541 atttgcctag atttgtgatt ctaccaagtg gaatttttgg gaaaatatca atattccacg
     3601 atttttattc aaatcataac gtttcgaaat tatcagctta aataaaacta aaataaataa
     3661 taaaatacta aaaaccctga atttctcggt tctcggttca gcttgagttc gttctataca
     3721 cgttatcgat ttttgtcatt tcgtttaatc atgggttttg attcatttta aatatgaaat
     3781 ttgatttatt cctcgtttcg aagttggttt gtcttgtccg gccggtaaac aaatgttatt
     3841 aaaaaaataa taaatttcga aatttttcgt aatgttccgc aatcgtgttt ttccgtttat
     3901 ctcgtcttcc cttctttcaa atatactatt tattttctca attcacatat tttcagccag
     3961 ccccgctcaa caagtggaat aaaaataact tcgtatagca tacattatac gaagttattc
     4021 tagaggatcc agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa
     4081 tgtccaaggg agaggaggac aacatggcca tcatcaagga gttcatgcgt ttcaaggtcc
     4141 acatggaggg atcagtcaac ggacacgagt tcgagatcga gggagaggga gagggacgtc
     4201 catacgaggg aacccaaacc gccaagctca aggtaagttt aaacatatat atactaacta
     4261 accctgatta tttaaatttt caggtcacca agggaggacc actcccattc gcctgggaca
     4321 tcctctcccc acaattcatg tacggatcaa aggcctacgt caagcaccca gccgacatcc
     4381 cagactacct caagctctcc ttcccagagg gattcaagtg ggagcgtgtc atgaacttcg
     4441 aggacggagg agtcgtcacc gtcacccaag actcctccct ccaagacgga gagttcatct
     4501 acaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtca
     4561 agctccgtgg aaccaacttc ccatccgacg gaccagtcat gcaaaagaag accatgggat
     4621 gggaggcctc ctccgagcgt atgtacccag aggacggagc cctcaaggga gagatcaagc
     4681 aacgtctcaa gctcaaggac ggaggacact acgacgccga ggtcaagacc acctacaagg
     4741 ccaagaagcc agtccaactc ccaggtaagt ttaaacatga ttttactaac taactaatct
     4801 gatttaaatt ttcaggagcc tacaacgtca acatcaagct cgacatcacc tcccacaacg
     4861 aggactacac catcgtcgag caatacgagc gtgccgaggg acgtcactcc accggaggaa
     4921 tggacgagct ctacaagtaa gaattcgcta gccggccata caagtaatcc ggatgatcga
     4981 cgccaacgtc gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat
     5041 ttatttattc tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct
     5101 aaacattttt ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac
     5161 aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg
     5221 gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa
     5281 cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg
     5341 aaggaaaact agttctagag cggccgcata acttcgtata gcatacatta tacgaagtta
     5401 ttctagagga tccagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     5461 aaatgtccaa gggagaggag ctcttcaccg gagtcgtccc aatcctcgtc gagctcgacg
     5521 gagacgtcaa cggacacaag ttctccgtct caggagaggg agagggagac gccacctacg
     5581 gaaagctcac cctcaagttc atctgcacca ccggaaagct cccagtccca tggccaaccc
     5641 tcgtcaccac cttcacttac ggagtccaat gcttctcccg ttacccagac cacatgaagc
     5701 gtcacgactt cttcaagtcc gccatgccag agggatacgt ccaagagcgt accatcttct
     5761 tcaaggtaag tttaaacatt aattaatact aactaaccct gattatttaa attttcagga
     5821 cgacggaaac tacaagaccc gtgccgaggt caagttcgag ggagacaccc tcgtcaaccg
     5881 tatcgagctc aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     5941 tcagggaatc gacttcaagg aggacggaaa catcctcgga cacaagctcg aatacaacta
     6001 caactcccac aacgtctaca tcatggccga caagcaaaag aacggaatca aggtcaactt
     6061 caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagatccg
     6121 tcacaacatc gaggacggat ctgtccaact cgccgaccac taccaacaaa acaccccaat
     6181 cggagacgga ccagtcctcc tcccagacaa ccactacctc tccacccaat ccgccctctc
     6241 caaggaccca aacgagaagc gtgaccacat ggtcctcaag gagttcgtca ccgctgccgg
     6301 aatcacccac ggaatggacg agctctacaa gtaggaattc gctagccggc catacaagta
     6361 atccggatga tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt
     6421 ttttttccta ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa
     6481 ttcaatccaa atctaaacat ttttttttct ctttccgtct cccaattcgt attccgcagg
     6541 ccttaagtga gtcgtattac ggactggccg tcgttttaca cccgtgcagt tgaaattgaa
     6601 aatgagttaa agttggagtt tttattgaaa acagatttcc gtgtgattag tgtttttagc
     6661 gagtgtgaca ggacagcgaa aaaatataga aacaaggggg gaactgaaaa gcttaggaat
     6721 gcattgaaca tgagaagggg aaggggaagg aacaaactag acaggaatta ttggaattta
     6781 atcacatttg gagttttttt tctattcgac agaataatta tccagaacat ttttgtatta
     6841 aatatttatg catcatatga gtagtcggct ttgttgtgca tgacgagttt gttatcgacg
     6901 aaatagaagc tgtcagaacg agtctcgttt ggattgttga tcatgtcgtc cactgaaaaa
     6961 gagattagtc ttttgaattg tactttttag aataatgact cactgagttg ttgagagagt
     7021 tgagggaact catagatatg ttcacagttg tttcgtgaat tcggaataca gaatccgaat
     7081 tcaaagtcaa aacacttcag aaggcgatct ttgaagaagt gacgttcgat cattcggaaa
     7141 tgatggattg ggatgtctcc tactttgaat tccacagttg ctccgaccgt tttgagtttc
     7201 aaaaagtttg gagcaaatct ataacgcacg tatcttgccg actcctgtgg cgactcatca
     7261 ttctcctgat cgttctccgg tttggcgatc tcgaaaagca cttgctcagt gtccagatca
     7321 cggatttgga acttggtgaa ctcgatgtta tagatgttcg cagatgggga gcataagaat
     7381 cctaaattta tgttttaaac tgaaatccaa agggagcaag ataccttgag tgattcccgg
     7441 aagtgctaaa acgtcgttcg gagtgatttg agctttcttc gcaagctccg attccgttgt
     7501 gattccttgt tcggtgcttg gtggtggccg tggcatctgg aaatatggaa aagttcaaca
     7561 aaaagaaaag agaaaagaat gaaatcggat atcaagagtt agttgagcgg tttctctagt
     7621 tttctgagtc tcacctgcga cgggaaggtc gccgagccgg gtggaatcga tcgttgttgc
     7681 tcggctttca tatcggtttg gttggaagcg gctgaaaacg gaaagaagtg gaagaaggaa
     7741 aagagtgtgg tgtgacagga aaatggtaat tagagggtgc caaataacca gctatatttt
     7801 gttttttttt gaaaacattt ttaaaaagaa aaatacgata atgatatcag atggatttcc
     7861 ggaaaactgg tatgaaaaat ttcaaccttt ttgagtacat gtaatcaaaa tacactttgt
     7921 aaattatcat ttttattgaa actccaccat ttttctattt ataacgctaa taatttgaaa
     7981 aagaaaccta ttgcgaaccg cggggtgaat cccaaaaacg aatgcgtttt ggtggagtga
     8041 ttgattcgaa tcgaagaaga aaaagaagaa gacgtggaat agagagctca ctcttaaccg
     8101 agcagcacac accgacagaa aaaaaaatga aatgaatgag ggtcttcttc ttcttcttct
     8161 tcgaatgatt gacagaaatg ggaaaaagag gaagattgag aagggaaaaa ggaaggagaa
     8221 aagaagcaga agaagacgtc agagaggaga ggaacgagcg gaaaagcagc gggcgcaagt
     8281 catagaagta gcagagctgg ggagaagaag acactatcca agaaaggaat gacgagagag
     8341 tatgcaaagg ggtatagggt gcagacagaa taggaacaga ataacagatg atgagccaag
     8401 aagagttgaa aagggcgatg aatttgtcat gtaacttaat ttgggtcaat ttgagcatga
     8461 tgaattgaaa tcatcccttg ttgggagtta ataaccggtt tgttatcaga aaccctgtaa
     8521 tagaagggcg ccctaacttt gagccaattc atcccggttt ctgtcaaata tatcaaaaag
     8581 tggtcaactg acaaattgtt tttgatatta taataaacat tttatccgtt aacaattttc
     8641 gaatactttt tacaaggact tggataaatt ggctcaagcc tgcttttttg tacaaacttg
     8701 cactagtgac acacggccaa atttcttatt ttcctgctat tttctccaaa tatgattaaa
     8761 atttgttcat ttcaaaattg cctccaactt gttttggggc aaaaatttca caaaacttca
     8821 cagcaaaatg tgaaacaata ttgctatcta ttgaaaacat taatgatagt cgagtgactt
     8881 cttgaaccaa tactcatcgc atcaaatgcc ttttctcgaa tactctgtaa taattggcat
     8941 ataaaaacat acatactcat cagtttgaaa gctaaccttt accataatca ttactaaaat
     9001 tggatttaaa gccggacacc aagaaaaaag atacggtaga taaactgaca gatgagaagt
     9061 ctcgatacga aataatggca ttagaaaaga cacatagaat ggaatgtgga tagtgagaaa
     9121 tggtagaatg aactgaaaaa ttgagaattt aaggagaaat gttattttta agctgctttg
     9181 cttgaaactt tgaatttaaa attttagaac aattactgtt tcaaaatatt tttattttta
     9241 tttactccat ttgaaaaaaa agcaacattg cttgttcaat ttctgttatt gccttacctg
     9301 aaccataagt accatattca attgttgttg agacttcatc gaatttgtac tccgacttcc
     9361 cttatgaatt tcgtatctga taattgtcca acaaaccatc gcaattactg tagttatcac
     9421 tagaacagcc attatataaa tcgtacatgc ccatgaaatt gggtcagcaa caaagtcatt
     9481 caacgtgtaa tcatatcttt ctagtgatgc aattaaatat gcagattttc cgattccatt
     9541 cagattgtat acgatatatt ctgatagttg atcgtcagca gcatctggga catagccaaa
     9601 ggtgattaga actgtggata ttagagagac aataaatgat atgattgtgg ttgtaaggac
     9661 cacgtttttt tgtgttctcg ttgttctgaa agtataattt gtagattctt aaactcagaa
     9721 tttatcatat ttgaactccg ctgtttttga attaagtaat agctgaaaca gtgaacatta
     9781 gtttaaaaat ataattttac aggcacgcac aaacttcggt gtgggtacgt attacctgcc
     9841 tcctattgaa tattcattgt ttaaacgttt tcagaaaaaa agtgtacatg ttttttggag
     9901 tatgatgatg atgtagtcgc aaggcagata tgaaatgtag gtaacacagt gcacagatgc
     9961 gttttttgcc aacatcaagg gttgtaagta acttttcaga gaaaaacata tagaaatttt
    10021 gtttcaagta tcgtatcagt ttttttctga ttaacttata aaaagaactc ttcgacaatt
    10081 tgaatgctta atttaaaaaa attaagtcga atatttcagc atgccaaccc gggagcaact
    10141 tttctataca aagttgatag cttggcggat ccctggcgtt acccaactta atcgccttgc
    10201 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    10261 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
    10321 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
    10381 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    10441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    10501 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
    10561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
    10621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    10681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    10741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    10801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10921 tccaatg
//
