LOCUS       sequence-121933-         617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-GST-Smt3-NpuDnaEN102-CBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Guerrero F, Ciragan A, Iwai H
  TITLE     Tandem SUMO fusion vectors for improving soluble protein expression 
            and purification.
  JOURNAL   Protein Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019.
  PUBMED    26297996
REFERENCE   2  (bases 1 to 617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Aug 20. pii: S1046-5928(15)30039-5. doi: 
            10.1016/j.pep.2015.08.019."
COMMENT     Sequence Label: pLJSRSF3
FEATURES             Location/Qualifiers
     source          1..617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttaataagg agatatacca tgggcagcag ccatcatcat catcatcacg gctcccctat
       61 actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct
      121 tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa
      181 aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa
      241 attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg
      301 ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata
      361 cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag
      421 caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa
      481 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata
      541 catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga
      601 agctatccca caaattg
//
