LOCUS       sequence-121937-       17531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and 
            UTR, for integration on cxtTi10816, Mos Chr IV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17531)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 17531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR11
FEATURES             Location/Qualifiers
     source          1..17531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      834..1422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1323..1342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1559..1579
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2999..3023
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3030..3046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3030..3046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(3985..4018)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4047..4067
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(11972..12005)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             12034..12054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(12067..12369,12423..12497,12549..12668,12720..12935)
                     /codon_start=1
                     /product="monomeric enhanced GFP, optimized with synthetic 
                     introns for expression in C. elegans"
                     /label=mEGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(13167..13184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13167..13183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             15124..15135
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(15281..15305)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(16740..16760)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(16964..16983)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17083..17105
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(17143..17161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17229..17333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17334..17402,17403..17531,1..663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
       61 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      961 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1441 tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccat cgaattatca
     1561 actatgtata ataaagttgc ggtacccaag gcgacaacgt aagtgactga aggcattaaa
     1621 ggtacatgcg tccgcgcaag tgtccgcttt tgctggccta acaagtaaga gaaacaagta
     1681 agaaagagta agagaaaact cacccgaggt gtctcttcta gcaaccgcct attcagataa
     1741 ttttaagcca gttcgagagt tatttgtcgc atggtataga agctcgttat ttacattaaa
     1801 tttttagtct tcccgcagaa gtggacgatg cgatgggcta cttcaaaaat ctgacgccac
     1861 gtgtcaagga ccttcacaag agcatgacaa acttggaaga tattagcaag tggcagaaga
     1921 aggccagttt ctctggcacc cttgagaatt actcgcgtct cggtgacaag atcaatgtga
     1981 aaccatttat ggatgctgtt gatgctagaa tgggtgccga agctgatgcc gtgaaagggg
     2041 tataaagcta ctgttgcacg acgactcaca gactaatcat tccaggtcct cgcgatttgt
     2101 gaaaaataca agtcattcta ccaaaacgag ggaaaacttc acgcggacag tatcgccaat
     2161 ttgaatagga ctcggctcga catggacagt gcggcggata aatatgcgaa caacgagact
     2221 gaagttaaca agactcgttt ggataacagt accacggaat ttgaagtggc ttgtgagagg
     2281 tttgtaggtc tttcgtaggc acgatatcct tatttgaatg tgtttcagaa tgcgagaact
     2341 ggcgaacgga atcaagacaa ttgaatcgaa ccattcttcc tggcaagacg gtcttatgaa
     2401 ggagattaaa gtggcgttgc gtaaataaag aaaggatcct ctttgaaaat aaagattttc
     2461 tacattcact tattttttta gctttatact tttcagatcg aatgaactgt ttccaacgtt
     2521 tccatattaa ttttttttag ttaaaaaagt tgggtattaa cagttttgtt aacagttttg
     2581 tcttccgaca gatgtgagac ggaaaaatgg ccaaaattcg agattttgac taaaatcagt
     2641 gtagcttttt caaaattttg aaccggcatg aaaaagtttg aaaagttttc gagaagtttc
     2701 aatccgttca tttgagccca tgttgggtct ataagatcaa aatcgcccga actgttagtc
     2761 ctcctttaag ttacgattat taacaaaatg tcgcctaagt ttagtttcac acttgtctca
     2821 cactagtcga tgaactgtgt gactactgta gtgagtacag tagggcccgt gtaaaaatac
     2881 aaaaaccaag aaaaagtaaa ccaaaataaa aaacgaattt attgttcact acaaccaaac
     2941 tgcaaagttc ttttcttacc catcagctca atcgtgtact tgcgtggaaa ccctaggagc
     3001 cactttgtac aagaaagctg ggtcacgggc aggaaacagc tatgaccatg attacgccaa
     3061 gctacgtaat acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc
     3121 tcgaggaatt cctgcagctg cagttcaatc ggtttttcct tgcttgcgcc caattctgat
     3181 ggttcttcca tttctaaatt ttagtttaat aaaaaaattt taaaataata cgagttttga
     3241 gactaacttt caataaaaac caaaaaaaat tgcttcaaaa aagagttcgc gccgatttac
     3301 gataaacaca gcgcgctacg acactccgaa aaccgcgccg caccattaca ttctccgccc
     3361 caccgcacgg tttgcggaag gcgatgaaat tgctgggcct aaaattttaa cccttttttt
     3421 cccgtttttc aactgttttc ccgatttttt cgatattttt ttcaggtttt tagttagaaa
     3481 agtatataaa aattgcatta tttgtagttt tcgctcgtaa tttgtgatag aaaacggcca
     3541 atttgcctag atttgtgatt ctaccaagtg gaatttttgg gaaaatatca atattccacg
     3601 atttttattc aaatcataac gtttcgaaat tatcagctta aataaaacta aaataaataa
     3661 taaaatacta aaaaccctga atttctcggt tctcggttca gcttgagttc gttctataca
     3721 cgttatcgat ttttgtcatt tcgtttaatc atgggttttg attcatttta aatatgaaat
     3781 ttgatttatt cctcgtttcg aagttggttt gtcttgtccg gccggtaaac aaatgttatt
     3841 aaaaaaataa taaatttcga aatttttcgt aatgttccgc aatcgtgttt ttccgtttat
     3901 ctcgtcttcc cttctttcaa atatactatt tattttctca attcacatat tttcagccag
     3961 ccccgctcaa caagtggaat aaaaataact tcgtatagca tacattatac gaagttattc
     4021 tagaggatcc agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa
     4081 tgtccaaggg agaggaggac aacatggcca tcatcaagga gttcatgcgt ttcaaggtcc
     4141 acatggaggg atcagtcaac ggacacgagt tcgagatcga gggagaggga gagggacgtc
     4201 catacgaggg aacccaaacc gccaagctca aggtaagttt aaacatatat atactaacta
     4261 accctgatta tttaaatttt caggtcacca agggaggacc actcccattc gcctgggaca
     4321 tcctctcccc acaattcatg tacggatcaa aggcctacgt caagcaccca gccgacatcc
     4381 cagactacct caagctctcc ttcccagagg gattcaagtg ggagcgtgtc atgaacttcg
     4441 aggacggagg agtcgtcacc gtcacccaag actcctccct ccaagacgga gagttcatct
     4501 acaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtca
     4561 agctccgtgg aaccaacttc ccatccgacg gaccagtcat gcaaaagaag accatgggat
     4621 gggaggcctc ctccgagcgt atgtacccag aggacggagc cctcaaggga gagatcaagc
     4681 aacgtctcaa gctcaaggac ggaggacact acgacgccga ggtcaagacc acctacaagg
     4741 ccaagaagcc agtccaactc ccaggtaagt ttaaacatga ttttactaac taactaatct
     4801 gatttaaatt ttcaggagcc tacaacgtca acatcaagct cgacatcacc tcccacaacg
     4861 aggactacac catcgtcgag caatacgagc gtgccgaggg acgtcactcc accggaggaa
     4921 tggacgagct ctacaagtaa gaattcgcta gccggccata caagtaatcc ggatgatcga
     4981 cgccaacgtc gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat
     5041 ttatttattc tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct
     5101 aaacattttt ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac
     5161 aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg
     5221 gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa
     5281 cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg
     5341 aaggaaaact agttctagag cggccgcttt ttctagagtt attttcgagc gatttattta
     5401 aagcgagcgc aatttgtctg atgggcaatt aattctaaat caaaatattg atattaatcc
     5461 atatatgtac gcacctcgta taagggtcac ccacttaacc gggtcacctc ctctcaccaa
     5521 ttggtgacat ggtttggatt gatgggtggt tggtttcgaa ggacggactc ctccttcacc
     5581 acaccccacc ctattccatt ctaccagtca gtacttctcc tcttcttcct cccatccgtc
     5641 cctgaatcga tactagggtc tcttcataca ctcggcgcgg tgttcctctg cacactcatc
     5701 accttactca ctatttcgcg aatcctttct cttcggcacg tcagatacgc agagaattga
     5761 agaaatggga ggtctcgggt gctgtattgc agctgctgct gggaaagaga caggcagttc
     5821 gttgcctctg cgtctctcca agctactgtt cgctatgaga tatttatgtg aaagtgttca
     5881 acagttttcg atttttgtac ggctttccat actttttttc aaaatgaaac tatccacttt
     5941 attttccatt ttcaaaggaa aactcctcct tcgtaaattc ttatttcata attgattgtc
     6001 caagtaaaaa gtaattgtta aataacattg aaataaattg aaacaaaagt ttttttaaaa
     6061 gtgtcatctt ttttcaataa aatcccataa actcggctct ttcccacatg ttctgcaatt
     6121 cacatgacat aaacatgaag ctcgagtcag agcacgtgtt ttctgtcgaa cacgaaattt
     6181 tttgagtgcc tcggataaat ttttcttttg aaaaaaacaa cttttgtata ataaaaccgg
     6241 tttgaaattt cccatcgaat ccacgaactt tcggcatttt atggcatatt ttctcttttc
     6301 tttttcgctt tcaaaccgaa caaatcatat taaaaacata tacgcataca caaaaatatg
     6361 tatgttgtat gtttttatgt tcacgatttt ccgtatagtt gtcgtagtag ttacgagcaa
     6421 attttgaaaa ttccgaaatg ttgcggctct gaatcgtgga atgcgaacaa agagaacaat
     6481 gccttgtact ttttaatatc gcagtttggg gaaatttccc caattttcac cttatcacat
     6541 cgctttatta cttcgtttct gtttctgttt gcagtcaatc agtcattgca aacgtctgat
     6601 tctcttctta tcatgaatct cttggtttga agagaggggt tctatcattg ttaagtaagt
     6661 tcgcttttcc gtatgatgtc tcgaagatta agacggccgt cattagttga aaagtctcct
     6721 gattttcact ttatccccaa taaaatgcta aaattttaca aacgtttagt ttaagcccaa
     6781 ttaacgaata gatcgatagc tttattcgtc acttgtcaac gctgaaggtt gcggctagat
     6841 gcataaaaag atatgcatct ttgtatctct cggaatcagc aattaggcat ctctcttctt
     6901 ctcttctcca aatgtttctc gaatagatgt attcgatagg cttgattgga ataaggtgag
     6961 actttttgaa tggagaggtg tttgaaggtc gcttggtgat ggttcaaaaa gttaggcttg
     7021 gttggttttt cagaattcat tattcaaata gatttttaaa aaatccactt tggcatattt
     7081 tcagcttcag atttcgctgc catgaacttt tcaacaaaaa aattataaga tgccaaaata
     7141 gttcttctgt agcgttccca cttttactta tcaataaact aaacgagtat tcaataaaag
     7201 ttgatgacca cgcacacaaa tcacacaata aaccaaacaa taattgataa cggtcatcat
     7261 tttgtaatag tgacctcccc caatcggggt acctatgtat cctctatctg tcaccacttt
     7321 ttcataagat gagttagttg acctgacatg gtctctattt gtcctcttcc tagtgtctcc
     7381 tctccattct cttagccggt catcattatg tggccttgtg tgtggtgcat agggacactg
     7441 ttaataattt tccatgtaca tatattttat attctttcca tgctttctct tctcctcatg
     7501 caactcgctt tattaaatat taatagctct cgttttttta caggcttttg cgtgttctcc
     7561 tcaactttac ccttttgcac ataaaatgga tgagcagcaa ccgccagcca actctccggc
     7621 tatttttcat tcgccacaag cgatgccggt gagacgaacc ctgggaccac acaactcacc
     7681 cgttaaatca atgtccacaa actcatcggt aagaaatttt ttacattttg gaaaaaatta
     7741 aaaaaaaatt tcaggctcat acatcgccac gagtcacacc tacaaaagga gcgaatcctt
     7801 acggggatcg atttatgcct ctacgtcaat caaatataga atggaatgct cgctatcatt
     7861 cgatcaactc ggatgatgac tcaggattca aaaaaccaat gggcacatca tcctgtaatc
     7921 ggcatttggg cggtgggcag aattctgcat tcatgtctga ttcgtcaacc catccaagtt
     7981 catcttctct cgctggatta ggtgctctcg caactgctcc atcttcttct cagtcatcac
     8041 tctcatcaca tccacatcaa catcataatc atcattcgtc gtcaagttca tcttatcatg
     8101 attattatca aggaacgtca tctggatcag gaaatacttc tccaacagct caagcagctt
     8161 gtatggatga caccagaagt agtgaagccg tcctcagagc ccttctacga aacgaaatgc
     8221 tgaaagatcg aattgatgac gtcaaaacgc aagttcaaac ttccgaagaa gttattgcca
     8281 cgttttcctt gggtcctgtc gtctctgctt tcccacagcc cactcaagaa tccggtacac
     8341 gtggaattat accacaattg gaacggaatg catacggtgg aagtgttgat acagtattcc
     8401 cagtagttgc caacgaagga attattgatg ttacgaggtg agattttaat ggttttcaaa
     8461 gtttttgttt aattttgaat tgaaaataag aattatagat ccgaaaaaaa agtttttttt
     8521 ctgttccaat attttccgtt aaaagttagt ttgaaaatat agaaaatcac ctgatttatc
     8581 aaaaaataac atttattcca gtgcactgga gttcatgaaa gtgctcaaaa atagcctgaa
     8641 aagccaaaaa ttccaaaaaa ttaaaaacaa agctagatag ttcgataatg ggtaattggc
     8701 aacttacagg tcatgtttga gcacattcgc tgaaataaat attattttca gataaatcgt
     8761 gttttttttt aaatttttga cactaatatt taaagaaacg ttgaaaataa ttgccgccca
     8821 tccccgatac ttgtttttaa tttttaattg taaaatttaa tgatattaaa aaaattttaa
     8881 ataaataaaa ttttcatgat ttacatttat ttcaacttta attttcagaa tcagtggtgc
     8941 agaatctcct acaatggccc aaatgatgga acctcgtctt cgctgtggtg ctgactccgt
     9001 agctgatatt cctttatcac cagctgccag tatcaacggg gatacaccaa caaaagcccc
     9061 accaccagct cttccattat cgccaattgt tcaaaaacaa tcgccagctc gatcactttt
     9121 tacgtacagt gctaaaacga cgcctgttaa atacggaggc caagcaacaa ctacagcgac
     9181 ttctccattt ggtggaccat ttggagttga ttcgcaacgt ttgttgagga ctccaaggaa
     9241 gccaattcga aaagttccaa agaatccgta taaagttttg gatgctccgg aacttcaaga
     9301 tgatttttat ttgaatttgg tcgattggtc atcacagaat caactttctg tgggattggc
     9361 agcttgtgtt tatttatgga gtgccacaac gagccaggtt ggattttaaa aaagattttc
     9421 aaaaaaaaaa tcgaaaattc tacaaaacta cctattgagc catgttattc ctatttttcc
     9481 attaaaaatt aatgtcggat taaaaaaaat attggattta acagaaaata agaaaaacaa
     9541 ttgaatgaag catatgcaaa agttctaaaa aatggtctga aagttgtcaa taaccgatta
     9601 tcatttttat cgaaattttg ttttcggcag tttgcaactc tcatgccatt ttcgagcatt
     9661 tttttcaaat gattcagtta aattaattta attttcaaat attcttcttt attttggcaa
     9721 tgaacgttgg agaaaaatga tggaacaaaa ttctaaaaaa atcataattt agtagaattg
     9781 catcccatct ccgtttacgg aaaattttca aaaaaaaatt gaaaaattcg agtttaaaaa
     9841 tactatgttt atttcacttt ttaataaatg attagcacat ttacctcaaa ttatcttgca
     9901 atttgtacaa ctttcaggtg ataaagcttt gcgatttggg tcaaacaaat gagcaggatc
     9961 aagtgacatc tgttcaatgg tgtgataaag gagatctttt ggcagttgga acaagtcgag
    10021 gtgtcaccca aatctgggat gtaacgactc agaaaaagac acgagagctc actggacact
    10081 cgtcaagagt cggttgcttg gcatggaatg ctgatacaat ttgctctgga tccagggatc
    10141 gtaccattat gcatcgtgat attcgatgtg atgataatga tatgggtaga aagttgacga
    10201 atcacagaca agaagtttgt ggattgaagt ggtcacctga taagcaactt ctcgcatccg
    10261 gtggaaatga taatcaatta cttgtctgga atctgagaag aaacgagcca attcagacat
    10321 acactcagca caatgcagct gtgaaagcgc tcgcgtggtc gccacatcat catggacttc
    10381 ttgtttctgg tggaggtact gctgatcgat gcttgagatt ctggaatacg ttgactgcgc
    10441 agccgatgca atgtgttgat actggatcac aggttaatat ttttaataat tgagcggata
    10501 atatgatttt aaatttctaa aacgaaaaaa tctgacgata acctaaatat ttaaattcta
    10561 aaatctgaaa aacgtagtat acaattttag aacatataca tgaattttca aagcttactt
    10621 ttttttttaa cttaaattta tattattttc aggtttgcaa tgtggcatgg tcgaagcata
    10681 gcagcgagct cgtctctacg cacggttatt catttaatca tgtaagttgt ttgcgaaaaa
    10741 ttttaaattt tccgtagaaa ttcgaataat tcaaaaacag tgtttaatga attcttaagc
    10801 ttcaggtaat catctggaaa tacccaagtc ttcagcctgt aaccaagttg gttgggcatc
    10861 agtatcgtgt attgtacctt gcaatgtcac cagatggcga atcgatcgta actggagccg
    10921 gtgatgagac tcttcgattc tggcacgtct ttaataaggg aaatccaccg acgatcacca
    10981 gatcaaaatt gaatttgcat tctaccatcc ggtagaattt cattttcaca tgttttttgt
    11041 tttttttttc tgaagtgatt gttttccaag aaaagtgcga ctatattaat agaaaatgtt
    11101 ttcaatttct gttaatgtat tttgtgcctt tttaaagaaa attattacct ccctactcat
    11161 caccttcctc tggtcaaaat tgccaaaact gggttaccat ttttcaaaaa aatcaagata
    11221 atctctagga ttttcgaatt gtatcttcac attgtttgtc acgattattc aaaaaattca
    11281 ctttatatgc tcgttttctc aatttctgta ctccctccaa atcataccat gcatctagtc
    11341 cccattgaaa tagtcgcctt attacaaaat tgtcaattcc tgagttacgg atttcacgag
    11401 tttgtaataa tatgaaatcg cccctctcgt ctcctgaaaa actatatgct gtaaaaattg
    11461 ggattcatct ccaaccactt tgcctagaat ttctagttct tcttttttgt tgctatgtgc
    11521 tcctgtgaat tcatcatcat gtaatttcaa tcgaagttcg gctttttcgc tgaacacccc
    11581 accccaaata agtctttcaa aaactttcaa tcgaatccta catgtttctt ttttcgccta
    11641 atatttaaaa ttattgctcc agtctgctta ttttttcccc cactgctccc agtgcttcta
    11701 cattttttgt tccaaatccc caacccctcc ccaagttttt ttttattttt acaaatttct
    11761 tccagctttt tatttgtctg cgacataata tatattttat taaactgata tttcctagaa
    11821 tgaaaaaaca aataaaactc gtttgtttga gaaaaaagta atttatatac agaaattaga
    11881 aaattttatg ggttttttaa atttttagaa gacgaatcgg cacataaatt ccaaattctt
    11941 agcttcttca atcgaattca ctcgcggccg cataacttcg tatagcatac attatacgaa
    12001 gttattctag aggatccaga aaaaatgact gctccaaaga agaagcgtaa ggtaccggta
    12061 gaaaaaatgt ccaagggaga ggagctcttc accggagtcg tcccaatcct cgtcgagctc
    12121 gacggagacg tcaacggaca caagttctcc gtctcaggag agggagaggg agacgccacc
    12181 tacggaaagc tcaccctcaa gttcatctgc accaccggaa agctcccagt cccatggcca
    12241 accctcgtca ccaccttcac ttacggagtc caatgcttct cccgttaccc agaccacatg
    12301 aagcgtcacg acttcttcaa gtccgccatg ccagagggat acgtccaaga gcgtaccatc
    12361 ttcttcaagg taagtttaaa cattaattaa tactaactaa ccctgattat ttaaattttc
    12421 aggacgacgg aaactacaag acccgtgccg aggtcaagtt cgagggagac accctcgtca
    12481 accgtatcga gctcaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
    12541 attttcaggg aatcgacttc aaggaggacg gaaacatcct cggacacaag ctcgaataca
    12601 actacaactc ccacaacgtc tacatcatgg ccgacaagca aaagaacgga atcaaggtca
    12661 acttcaaggt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
    12721 tccgtcacaa catcgaggac ggatctgtcc aactcgccga ccactaccaa caaaacaccc
    12781 caatcggaga cggaccagtc ctcctcccag acaaccacta cctctccacc caatccgccc
    12841 tctccaagga cccaaacgag aagcgtgacc acatggtcct caaggagttc gtcaccgctg
    12901 ccggaatcac ccacggaatg gacgagctct acaagtagga attcgctagc cggccataca
    12961 agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt
    13021 tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa
    13081 ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat tcgtattccg
    13141 caggccttaa gtgagtcgta ttacggactg gccgtcgttt tacacccgtg cagttgaaat
    13201 tgaaaatgag ttaaagttgg agtttttatt gaaaacagat ttccgtgtga ttagtgtttt
    13261 tagcgagtgt gacaggacag cgaaaaaata tagaaacaag gggggaactg aaaagcttag
    13321 gaatgcattg aacatgagaa ggggaagggg aaggaacaaa ctagacagga attattggaa
    13381 tttaatcaca tttggagttt tttttctatt cgacagaata attatccaga acatttttgt
    13441 attaaatatt tatgcatcat atgagtagtc ggctttgttg tgcatgacga gtttgttatc
    13501 gacgaaatag aagctgtcag aacgagtctc gtttggattg ttgatcatgt cgtccactga
    13561 aaaagagatt agtcttttga attgtacttt ttagaataat gactcactga gttgttgaga
    13621 gagttgaggg aactcataga tatgttcaca gttgtttcgt gaattcggaa tacagaatcc
    13681 gaattcaaag tcaaaacact tcagaaggcg atctttgaag aagtgacgtt cgatcattcg
    13741 gaaatgatgg attgggatgt ctcctacttt gaattccaca gttgctccga ccgttttgag
    13801 tttcaaaaag tttggagcaa atctataacg cacgtatctt gccgactcct gtggcgactc
    13861 atcattctcc tgatcgttct ccggtttggc gatctcgaaa agcacttgct cagtgtccag
    13921 atcacggatt tggaacttgg tgaactcgat gttatagatg ttcgcagatg gggagcataa
    13981 gaatcctaaa tttatgtttt aaactgaaat ccaaagggag caagatacct tgagtgattc
    14041 ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc tccgattccg
    14101 ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat ctggaaatat ggaaaagttc
    14161 aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag agttagttga gcggtttctc
    14221 tagttttctg agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa tcgatcgttg
    14281 ttgctcggct ttcatatcgg tttggttgga agcggctgaa aacggaaaga agtggaagaa
    14341 ggaaaagagt gtggtgtgac aggaaaatgg taattagagg gtgccaaata accagctata
    14401 ttttgttttt ttttgaaaac atttttaaaa agaaaaatac gataatgata tcagatggat
    14461 ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt acatgtaatc aaaatacact
    14521 ttgtaaatta tcatttttat tgaaactcca ccatttttct atttataacg ctaataattt
    14581 gaaaaagaaa cctattgcga accgcggggt gaatcccaaa aacgaatgcg ttttggtgga
    14641 gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg gaatagagag ctcactctta
    14701 accgagcagc acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt cttcttcttc
    14761 ttcttcgaat gattgacaga aatgggaaaa agaggaagat tgagaaggga aaaaggaagg
    14821 agaaaagaag cagaagaaga cgtcagagag gagaggaacg agcggaaaag cagcgggcgc
    14881 aagtcataga agtagcagag ctggggagaa gaagacacta tccaagaaag gaatgacgag
    14941 agagtatgca aaggggtata gggtgcagac agaataggaa cagaataaca gatgatgagc
    15001 caagaagagt tgaaaagggc gatgaatttg tcatgtaact taatttgggt caatttgagc
    15061 atgatgaatt gaaatcatcc cttgttggga gttaataacc ggtttgttat cagaaaccct
    15121 gtaatagaag ggcgccctaa ctttgagcca attcatcccg gtttctgtca aatatatcaa
    15181 aaagtggtca actgacaaat tgtttttgat attataataa acattttatc cgttaacaat
    15241 tttcgaatac tttttacaag gacttggata aattggctca agcctgcttt tttgtacaaa
    15301 cttgcactag tgacacacgg ccaaatttct tattttcctg ctattttctc caaatatgat
    15361 taaaatttgt tcatttcaaa attgcctcca acttgttttg gggcaaaaat ttcacaaaac
    15421 ttcacagcaa aatgtgaaac aatattgcta tctattgaaa acattaatga tagtcgagtg
    15481 acttcttgaa ccaatactca tcgcatcaaa tgccttttct cgaatactct gtaataattg
    15541 gcatataaaa acatacatac tcatcagttt gaaagctaac ctttaccata atcattacta
    15601 aaattggatt taaagccgga caccaagaaa aaagatacgg tagataaact gacagatgag
    15661 aagtctcgat acgaaataat ggcattagaa aagacacata gaatggaatg tggatagtga
    15721 gaaatggtag aatgaactga aaaattgaga atttaaggag aaatgttatt tttaagctgc
    15781 tttgcttgaa actttgaatt taaaatttta gaacaattac tgtttcaaaa tatttttatt
    15841 tttatttact ccatttgaaa aaaaagcaac attgcttgtt caatttctgt tattgcctta
    15901 cctgaaccat aagtaccata ttcaattgtt gttgagactt catcgaattt gtactccgac
    15961 ttcccttatg aatttcgtat ctgataattg tccaacaaac catcgcaatt actgtagtta
    16021 tcactagaac agccattata taaatcgtac atgcccatga aattgggtca gcaacaaagt
    16081 cattcaacgt gtaatcatat ctttctagtg atgcaattaa atatgcagat tttccgattc
    16141 cattcagatt gtatacgata tattctgata gttgatcgtc agcagcatct gggacatagc
    16201 caaaggtgat tagaactgtg gatattagag agacaataaa tgatatgatt gtggttgtaa
    16261 ggaccacgtt tttttgtgtt ctcgttgttc tgaaagtata atttgtagat tcttaaactc
    16321 agaatttatc atatttgaac tccgctgttt ttgaattaag taatagctga aacagtgaac
    16381 attagtttaa aaatataatt ttacaggcac gcacaaactt cggtgtgggt acgtattacc
    16441 tgcctcctat tgaatattca ttgtttaaac gttttcagaa aaaaagtgta catgtttttt
    16501 ggagtatgat gatgatgtag tcgcaaggca gatatgaaat gtaggtaaca cagtgcacag
    16561 atgcgttttt tgccaacatc aagggttgta agtaactttt cagagaaaaa catatagaaa
    16621 ttttgtttca agtatcgtat cagttttttt ctgattaact tataaaaaga actcttcgac
    16681 aatttgaatg cttaatttaa aaaaattaag tcgaatattt cagcatgcca acccgggagc
    16741 aacttttcta tacaaagttg atagcttggc ggatccctgg cgttacccaa cttaatcgcc
    16801 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    16861 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
    16921 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
    16981 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    17041 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    17101 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
    17161 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
    17221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    17281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    17341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    17401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    17461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    17521 gttttccaat g
//
