LOCUS       sequence-121938-        9654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of murine PDGFRbeta tagged with GFP under the control of 
            EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Nielsen BS, Malinda RR, Schmid FM, Pedersen SF, Christensen ST, 
            Pedersen LB
  TITLE     PDGFRbeta and oncogenic mutant PDGFRalpha D842V promote disassembly 
            of primary cilia through a PLCgamma- and AURKA-dependent mechanism.
  JOURNAL   J Cell Sci. 2015 Oct 1;128(19):3543-9. doi: 10.1242/jcs.173559. Epub
            2015 Aug 19.
  PUBMED    26290382
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.173559"; journalName: "J Cell Sci"; date: "2015-10-1- 
            1"; volume: "128"; issue: "19"; pages: "3543-9"
COMMENT     Sequence Label: pcDNA5FRT-EF-Pdgfrbeta-EGFPN
FEATURES             Location/Qualifiers
     source          1..9654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        247..1429
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          478..1420
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1377..1397
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4835..4844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4841..4843,4844..4846,4847..5560)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4841..4843,4844..4846,4847..5557)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4886..4907)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5147..5166)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5494..5515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5606..5623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5612..5836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6120..6167
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6175..7197
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6175..7197
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7327..7448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7364..7383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7418..7437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7497..7513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7497..7513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7510..7532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7521..7537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7545..7551,7552..7569,7570..7575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7590..7611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7728..7745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7899..8487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7979..7998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8658..9449,9450..9518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9281..9300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9519..9623)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtaggagtg
      241 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      301 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
      361 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      421 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
      481 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
      541 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
      601 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
      661 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
      721 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
      781 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
      841 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
      901 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
      961 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     1021 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     1081 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     1141 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     1201 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     1261 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     1321 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     1381 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattctcta
     1441 gagatccctc gacctcgaga tccattgtgc tggatctaca atggccccgc tagcgtttaa
     1501 acttaagctt atggggcttc caggagtgat accagcttta gtcctcagag gccagttgtt
     1561 gctgtccgtg ttatggctcc tgggaccgca gacctcccgg ggcctagtca tcacgccccc
     1621 tgggccagag tttgttctca acatctccag cacctttgtt ctgacctgct cgggctcagc
     1681 tccggtgatg tgggaacaga tgtcccaggt gccctggcaa gaagcggcca tgaatcagga
     1741 cggcaccttc tccagtgtgc tgacactgac caatgtcact gggggagaca ctggggaata
     1801 cttttgtgtc tacaacaact cactagggcc ggagctcagt gagaggaagc gtatctatat
     1861 ctttgtgcca gatcccacca tggacttcct ccctatggac tccgaggacc tgttcatttt
     1921 tgtcacggat gtcactgaga cgacaattcc gtgccgagtg acagaccccc agctggaggt
     1981 gacgctacat gagaagaaag tggatatccc cctacacgta ccctacgacc accagcgagg
     2041 tttcactggt acttttgagg acaagactta catttgcaaa accaccattg gggacaggga
     2101 agtggactcc gatacttact acgtctacag cctccaggtg tcatccatca acgtctctgt
     2161 gaatgccgtg cagactgtgg tccgccaggg cgagagcatc accatccggt gcattgtgat
     2221 gggcaatgat gtggtgaact tccaatggac gtacccccgc atgaagagtg ggcggctggt
     2281 ggagccagtg acagactacc tctttggagt gccctcccgc attggctcca tcctgcatat
     2341 ccccacggct gagctgagtg attcgggcac ctatacttgc aacgtgtcag tgagtgtgaa
     2401 cgaccatggc gatgagaaag ccatcaacat ctctgtgatc gagaatggct acgtgcggct
     2461 gctggagaca ctgggagatg tagaaattgc tgagctgcac cggagtcgga cgctgcgggt
     2521 ggtgttcgag gcttatccga tgccttctgt cctgtggctc aaggacaacc gtaccttggg
     2581 tgactccggc gctggcgagt tagttttgtc tactcgcaac atgtctgaga cccggtacgt
     2641 gtcagaactg atcctggtac gtgtgaaggt gtcagaagcg ggctactata ctatgcgagc
     2701 cttccacgag gacgatgagg tccagctctc cttcaagctg caggtcaatg tccccgtccg
     2761 tgtgctggag ctgagtgaga gtcaccctgc caatggggag cagacaatcc gctgtcgtgg
     2821 ccggggcatg cctcagccaa atgtcacctg gtctacctgc agagacctca aaagtaggtg
     2881 tccacgaaaa ctgtcaccca cacccttggg gaatagttcc aaggaggaga gccagctaga
     2941 aacgaatgtg actttctggg aggaagatca ggaatacgag gtggtgagca cactgcgcct
     3001 gcgccacgtg gatcagccac tgtccgtacg ctgcatgctg cagaactcca tgggtggaga
     3061 ttcgcaggag gtcaccgtgg tcccacattc cttgcccttc aaagtggtgg tgatctcagc
     3121 catcctggcc ttagtggtcc ttaccgtcat ctctctcatc atcctcatca tgctgtggca
     3181 gaagaagcca cgctatgaga tccgatggaa ggtcattgag tctgtgagct ctgacggtca
     3241 tgagtacatc tacgtggacc ctgtgcagtt gccttacgac tccacctggg agctgccacg
     3301 ggaccagctt gttctgggac gcactcttgg ctctggggct ttcggacagg tggtggaggc
     3361 cacagctcac ggtctgagcc attcgcaggc caccatgaaa gtggctgtca agatgctgaa
     3421 atcgacagcc agaagtagcg agaagcaagc cttaatgtcc gagctgaaga ttatgagtca
     3481 tcttggaccc cacctgaacg tggtcaacct gctgggggcc tgcaccaaag gagggcccat
     3541 ctacatcatc acggaatact gccgatacgg tgatctggtg gactacctgc accggaacaa
     3601 acacaccttc ttgcagcgac actccaacaa gcattgtccg cccagtgctg agctctacag
     3661 caacgccctg ccagtggggt tctccctacc cagccacttg aacctgactg gggagagtga
     3721 cggtggctac atggatatga gcaaggatga atctatagat tacgtgccca tgttggacat
     3781 gaaaggagac atcaaatacg cagacattga gtcccccagc tacatggccc cttatgataa
     3841 ctatgtccca tctgcccctg aaaggaccta tcgcgccacc ttaatcaacg actcaccagt
     3901 gctcagctac acagacctcg tgggcttcag ctaccaagtg gccaacggca tggacttctt
     3961 agcctctaag aactgtgttc accgagactt ggcggccagg aatgtgctca tctgcgaggg
     4021 caagctggtc aagatctgtg acttcggcct ggctcgagac atcatgaggg actcaaacta
     4081 catctccaaa ggcagcacct tcctgcctct gaagtggatg gccccagaga gcatcttcaa
     4141 cagcctctac accactttga gtgatgtctg gtcttttggg atcctactct gggagatctt
     4201 cacactgggt ggcacccctt acccagagct gcccatgaac gaccagttct acaatgccat
     4261 caagaggggc taccgcatgg cccagcctgc tcatgcctcc gacgagatct atgagatcat
     4321 gcagaaatgc tgggaagaaa agtttgagac tcgacccccc ttctcccagc tggtgctgct
     4381 cctggagagg cttctgggtg aaggctataa aaagaagtac cagcaggtag atgaggagtt
     4441 cctgaggagt gaccatcctg ccatcctgag gtcccaagcc cgctttccgg ggatccacag
     4501 cctccgatcc cctctggaca ccagctctgt tctctacact gccgtgcagc ccaatgagag
     4561 tgacaatgac tacatcatcc ccttacctga ccccaagcct gacgttgctg atgaaggtct
     4621 cccagagggg tcccccagcc ttgccagttc caccttgaat gaagtcaaca cttcctccac
     4681 catctcctgc gacagtcccc tggagctcca agaagagcca cagcaagcag agcctgaggc
     4741 acaactggag cagccacagg attcaggctg cccaggacct ctggctgaag cagaggacag
     4801 cttcctggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
     4861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     4921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5521 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgct cgagtctaga
     5581 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5641 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5701 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5761 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5881 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     6121 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     6181 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     6241 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     6301 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6361 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6421 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6481 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     6541 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     6601 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     6661 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     6721 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     6781 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     6841 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     6901 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     6961 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     7021 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     7081 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     7141 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     7201 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7261 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7321 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7441 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7501 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7561 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7621 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7681 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7741 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7801 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7861 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7921 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7981 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8041 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8101 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8161 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8221 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8281 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8341 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8401 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8461 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8521 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8581 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8641 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8701 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8761 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8821 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8881 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8941 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9001 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9061 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9121 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9181 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9241 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9301 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9361 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9421 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9481 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9541 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9601 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
