LOCUS       sequence-121943-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Terminator quantification vector utilizing Golden Gate Assembly and 
            amilCP. Positive colonies are green and false positives are blue. 
            Terminators are placed between GFP and RFP - induced by arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  TITLE     Reyna Lab - Terminator Verification Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PGR-Blue
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..27
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(55..339)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(113..132)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 tttacgcatc tagtatttct cctctttctc tagtagctag cccaaaaaaa cggtatggag
       61 aaacagtaga gagttgcgat aaaaagcgtc aggtaggatc cgctaatctt atggataaaa
      121 atgctatggc atagcaaagt gtgacgccgt gcaaataatc aatgtggact tttctgccgt
      181 gattatagac acttttgtta cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa
      241 tgcttttaat aagcggggtt accggtttgg ttagcgagaa gagccagtaa aagacgcagt
      301 gacggcaatg tctgatgcaa tatggacaat tggtttcttc tctgaatggc gggagtatga
      361 aaagtatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt aatgcccatc
      421 tggtggcggg tttaacgccg attgaggcca acggttatct cgattttttt atcgaccgac
      481 cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg gtggtgaaaa
      541 atcagggacg agaatttgtt tgccgaccgg gtgatatttt gctgttcccg ccaggagaga
      601 ttcatcacta cggtcgtcat ccggaggctc gcgaatg
//
