LOCUS       sequence-121944-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Terminator quantification vector utilizing Golden Gate Assembly and 
            amilCP. Positive colonies are green and false positives are blue. 
            Terminators are placed between GFP and RFP - induced by arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Reyna Lab - Terminator Verification Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PGR-Blue
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             177..188
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             262..273
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gcgaacggca gcggaccacc tttggtaact ttcagtttag cggtctgggt accttcgtac
       61 ggacgacctt caccttcacc ttcgatttcg aactcgtgac cgttaacgga accttccata
      121 cgaactttga aacgcatgaa ctctttgata acgtcttcgg aggaagccat ctagtatttc
      181 tcctctttct gcagccgctg agaccaaaaa gagtattgac ttcgcatctt tttgtaccta
      241 taatgtgtgg atctagtaat taaagaggag aaatactaga tgagtgtgat cgctaaacaa
      301 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
      361 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc
      421 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca
      481 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat
      541 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
      601 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc
      661 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt
      721 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt
      781 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg
      841 ta
//
