LOCUS       sequence-122023-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite Gateway destination vector to create transgenes for 
            phiC31-mediated transgenesis in zebrafish, contains an attB site and
            alpha-cystallin:YFP (CY) transgenesis marker in backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
  TITLE     Site-directed zebrafish transgenesis into single landing sites with 
            the phiC31 integrase system.
  JOURNAL   Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 
            Jul 3.
  PUBMED    23723152
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.23989"; journalName: "Dev Dyn"; date: "2013-08"; 
            volume: "242"; issue: "8"; pages: "949-63"
COMMENT     Sequence Label: pDestattB/CY (pCM327)
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    139..346
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     335..352
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(373..624)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(415..436)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     764..783
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggatc
       61 tagcggatcc gggaattggg aattcgttaa cagatctgcg gccgcggctc gagtacccac
      121 cgcggtggcg gcgatctctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
      181 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
      241 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
      301 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
      361 cgggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      421 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
      481 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      541 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
      601 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta
      661 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      721 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      781 gtccttgaa
//
