LOCUS       sequence-122025-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for Dre knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBGX
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    100..133
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(142..175)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcggc ctcggatccg cgatcgatcc gcggtgcggg tgccagggcg tgcccttggg
      121 ctccccgggc gcgtactcca cataacttcg tatagcatac attatagcaa tttatcgaag
      181 ttcctattcc gaagttccta ttctatcaga agtataggaa cttcaccggt gccaccatgg
      241 gcgcctccga gctgatcatc agcggcagca gcggaggctt cctgagaaac atcggcaaag
      301 agtaccagga agccgccgag aacttcatgc ggttcatgaa cgaccagggc gcctacgccc
      361 ctaacaccct gagggacctg agactggtgt tccacagctg ggccagatgg tgccacgcca
      421 gacagctggc ctggttcccc atcagccccg agatggccag agagtacttc ctgcagctgc
      481 acgacgccga tctggccagc accaccatcg acaagcacta cgccatgctg aacatgctgc
      541 tgagccactg cggcctgcct ccactgagcg acgacaagag cgtgtccctg gccatgagaa
      601 gaatcagaag agaggccgcc accgagaagg gcgagagaac cggccaggcc atccccctga
      661 gatgggacga cctgaagctg ctggacgtgc tgctgtccag atccgagaga ctggtggacc
      721 tgaggaacag agccttcctg ttcgtggcct acaacaccct gatgaggatg agcgagatca
      781 gcagaatcag agtgggcgac ctggaccaga ccggcgacac cgtgacc
//
