LOCUS       sequence-122029-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated 
            recombination into MIN-tagged cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-GFP
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(44..77)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        141..159
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     141..158
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(211..230)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             649..672
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             712..756
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             769..789
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    798..835
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct agtgtgcggc gcgataactt cgtatagcat
       61 acattatacg aagttatcgc tcgagtagga caaatccgcc gggataatta atacataacc
      121 ttatgtatca tacacatacg atttaggtga cactatagaa gcttaacatc cacttttttt
      181 tccccacagg agcgcaccat ctttttcaag gacgacggca actacaagac cagggccgag
      241 gtgaagttcg agggcgacac actggtgaac aggatcgagc tgaagggcat cgacttcaaa
      301 gaggacggca acatcctggg ccacaagctg gaatacaact acaacagcca caacgtgtac
      361 attatggccg acaagcagaa gaacggcatc aaggtcaact tcaagatccg gcacaacatc
      421 gaggacggca gcgtgcagct ggccgaccac taccagcaga acacccccat cggcgacggc
      481 cccgtgctgc tgcccgacaa ccactacctg agcacccaga gcgctctcag caaggacccc
      541 aacgagaagc gggaccacat ggtgctgaaa gaatttgtga cagccgccgg aatcaccctg
      601 ggcatggacg agctgtacaa gagcagcggc cccagcggca gcagcagcct ggaagtgctg
      661 ttccagggcc ccctgagcag ctctggcccc tctggcagcg ccgccaagtt caaagaaaca
      721 gctgctgcta aattcgagcg gcagcacatg gacagcggag gaggaggcga gaacctgtac
      781 tttcagggag gcggaccggc ttgtcgacga cggcggtctc cgtcgtcagg atcatccgtg
      841 tccaagggcg aggaactgtt caccggcgtg gtgcccatcc tggtggagct ggacggcgac
      901 gtgaacggcc acaagttcag cgtgtccggc gagggcgaag gcgacgccac ctacggcaag
      961 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctggtg
     1021 accaccctga cctacggcgt gcagtgcttc agcagatacc cc
//
