LOCUS       sequence-122030-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated 
            recombination into MIN-tagged cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-GFP
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(69..116)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    122..155
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    513..550
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             complement(559..579)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(592..636)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(676..699)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatgg ccgatcccat ggtttagttc
       61 ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacagaa
      121 tataacttcg tataatgtat gctatacgaa gttatcgagc ggccgcagta gcatagggtt
      181 tgcagacagc tctccttata tagaccggat cccaccgtac acacttacct ggacgtagcc
      241 ctcgggcatg gcgctcttga agaaatcgtg ctgcttcatg tggtcggggt atctgctgaa
      301 gcactgcacg ccgtaggtca gggtggtcac cagggtgggc cagggcacgg gcagcttgcc
      361 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcgtcgcctt cgccctcgcc
      421 ggacacgctg aacttgtggc cgttcacgtc gccgtccagc tccaccagga tgggcaccac
      481 gccggtgaac agttcctcgc ccttggacac ggatgatcct gacgacggag accgccgtcg
      541 tcgacaagcc ggtccgcctc cctgaaagta caggttctcg cctcctcctc cgctgtccat
      601 gtgctgccgc tcgaatttag cagcagctgt ttctttgaac ttggcggcgc tgccagaggg
      661 gccagagctg ctcagggggc cctggaacag cacttccagg ctgctgctgc cgctggggcc
      721 gctgctcttg tacagctcgt ccatgcccag ggtgattccg gcggctgtca caaattcttt
      781 cagcaccatg tggtcccgct tctcgttggg gtccttgctg agagcgctct gggtgctcag
      841 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      901 gtcggccagc tgcacgctgc cgtcctcgat gttgtgccgg atcttgaagt tgaccttgat
      961 gccgttcttc tgcttgtcgg ccataatgta cacgttgtgg ctgttgtagt tgtattccag
     1021 cttgtggccc aggatgttgc cgtcctcttt gaagtcgatg cccttcagct cgatcctgtt
     1081 caccagtgtg tcgccctcga acttcacctc ggccctggtc ttgtagttgc cgtc
//
