LOCUS       sequence-122059-       14615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB327
FEATURES             Location/Qualifiers
     source          1..14615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2302..2961
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2368..2387)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3303..3608
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3487..3506
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3649..3773)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3805..4500
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      4522..4774
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4808..4824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4808..4824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4821..4843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4832..4848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4856..4862,4863..4880,4881..4886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4901..4922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5007..5182
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7266..7942)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7309..7329)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8137..8161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8682..9473
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9719..10307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10208..10227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10461..10478
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10493..10633
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10559..10578)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10719..10741
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10977..11171
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11237..12310)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12739..13368)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
     2281 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2341 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2401 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2461 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2521 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2581 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2641 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2701 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2761 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2821 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2881 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2941 gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag ataacagtat
     3001 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     3061 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
     3121 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     3181 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     3241 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
     3301 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     3361 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     3421 gtctgctgtc agataaagtc ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     3481 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     3541 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     3601 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
     3661 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
     3721 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
     3781 agcgctggag gctccggtgg gagcatgagc gagctgatta aggagaacat gcacatgaag
     3841 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
     3901 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     3961 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac
     4021 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
     4081 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     4141 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
     4201 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
     4261 ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc
     4321 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
     4381 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     4441 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaagtga
     4501 gcttagagct cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg
     4561 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     4621 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     4681 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     4741 ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt
     4801 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4921 taggctgaat taggcgcgcc tatttctgaa ttcaacacat tgcggacgtt tttaatgtac
     4981 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg
     5041 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     5101 atatgctcaa cacatgagcg aaaccctata ggaaccctaa attcccttat ctgggaacta
     5161 ctcacacatt attatggaga aactcgaagt tagtatatag tccttccatc accctctgtg
     5221 ataatctcct taaaacttgc tcccccagga atcataggca gaacgtgctc ctggtgggga
     5281 actatcacat ccagtaaata aggaccggga gaatctagca tagtttgaat tgctgctctt
     5341 agttcatcct tcttcaccac acgcgcattt ggcaccttga aaccgtctgc aactgtacag
     5401 aagtcggggt agatctgctc ctcgtgatct ggatcaccta agtaagtatg tgctctgttg
     5461 gccttgtaga atctatcctc caattgtacg accattccta gatgttggtt gtttaagatc
     5521 atgatcttca cgggtaactt ctccacaaaa attgtagcca actcctgtac gttcatcaca
     5581 aagctaccat cgccatcgat gtcgatcaca ggaatatctg gttgagttgc ggcagccccc
     5641 aaagcagaag gaagtccgaa tcccatggct ccaagacctc cagatgtcaa ccaacgccgc
     5701 ggttcgttgt acttgaacca ctgggctgcc cacatttgat gttggccaac accagtagat
     5761 atgatggcat ttccaccagt gagttcgtac agcgtctgta tggcagattg aggagcaatg
     5821 gcatcctcga atacgggaaa cttcattggc catttctgct tttgttcttc tagttctgcc
     5881 acccagttgg agaagtcgaa agtcctcttt tgactcttca agagggcatt gatttcactt
     5941 agcgcaggtt tcacatcagc acacatggac acatggggct gtttgttctt tccaatttca
     6001 gcagggtcaa tatcgatatg aacgatgctg gacctgctag caaaagtctc cagtttacca
     6061 gtcactcgat cgtcgaaacg cactccaaat gccaacaaca gatctgcttg gtccacggcg
     6121 tagttggcgt aaacagttcc atgcattcca agcattttca aacttaatgg atcagactcg
     6181 ggatacgctc ccaaacccat taaagtcgat gtgataggaa ttccggtaag ctcgacaaat
     6241 tccctcaact cttcgctggc gtgattgcaa cctcctccag aatacactac aggcttctta
     6301 gaactagaaa tgagtcttat aatctgctcg attaacgttg gttccggtgg ccgaggcagt
     6361 cgttgtaagt agcccttcag ttgcattggc tgagaccaat ctggaatagc catttgctgt
     6421 tgcacgtcct tgggaatatc taccaagact gggcccggcc tacctgaagc tgccaaaaag
     6481 aaagcctccc tgattacgcg agggatgtca tcaatgttca taacaaggta attgtgcttg
     6541 gtgatcgatc tcgtaacctc cacaattgga gtttcttgaa aagcatcggt accaatcatc
     6601 ctactggata cttgacctgt aatggccact agtggcacac tgtccaagag ggcatcagcg
     6661 agcccggtaa caagattggt cgctccagga ccggatgtgg ctatacaaac ccctactcgt
     6721 ccagacgatt tagcataacc ttcagcagcg aaaatctccc cctgctcgtg gcgacagaga
     6781 atgttgcgga tgctgctgga acgtgtaaga gcctggtgaa tctccataga cgctccgccg
     6841 ggataagcga acaccgttct aatcccttct cgctcaagag cctccaccaa aatgtcggct
     6901 cctttgcggg gctcgtcatc cttgtagcga gactgaaagt cggacggggt tggtgcaacg
     6961 agtgacttcc cgctgctaag ccgtggtcca ctcacatcga ctgcagactg gatcttctcg
     7021 ctaacagctt gggctttcac ttccagtgaa cgctcagtct tccgagaggt gttgtatgca
     7081 tgtcccctca gagcggctcc cgagaagaaa ccactcctca cctttagctc tttagctccc
     7141 ttgcaacaag accttgccga tggctgcacc ttctccaggc tcgctatgct caaaggcaca
     7201 gctgcgcatc cactaattga cgccatgctc gagagagata gatttgtaga gagagactgg
     7261 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
     7321 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
     7381 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     7441 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     7501 ttatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     7561 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     7621 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     7681 tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     7741 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     7801 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     7861 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     7921 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     7981 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
     8041 aaaccgaatt cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt
     8101 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     8161 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     8221 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     8281 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     8341 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     8401 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     8461 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     8521 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     8581 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     8641 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     8701 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     8761 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     8821 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     8881 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     8941 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     9001 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     9061 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     9121 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     9181 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     9241 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     9301 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     9361 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     9421 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     9481 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     9541 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     9601 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     9661 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9721 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9781 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9841 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     9901 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9961 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10021 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10081 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10141 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10201 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10321 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10381 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10441 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
    10501 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    10561 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
    10621 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    10681 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    10741 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
    10801 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
    10861 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
    10921 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
    10981 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
    11041 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
    11101 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
    11161 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
    11221 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
    11281 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
    11341 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
    11401 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
    11461 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
    11521 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
    11581 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
    11641 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
    11701 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
    11761 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
    11821 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
    11881 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
    11941 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
    12001 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    12061 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
    12121 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    12181 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    12241 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    12301 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    12361 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    12421 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    12481 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    12541 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    12601 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    12661 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    12721 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    12781 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    12841 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    12901 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    12961 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    13021 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    13081 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    13141 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    13201 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    13261 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    13321 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    13381 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    13441 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    13501 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    13561 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    13621 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    13681 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    13741 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    13801 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    13861 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    13921 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    13981 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    14041 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    14101 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    14161 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    14221 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    14281 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    14341 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    14401 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    14461 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    14521 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    14581 ggcatgcaca tacaaatgga cgaacggata aacct
//
