LOCUS       sequence-122067-        4710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  phlh-8::tagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ruijtenberg S, van den Heuvel S
  TITLE     G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit 
            during Muscle Differentiation.
  JOURNAL   Cell. 2015 Jul 16;162(2):300-13. doi: 10.1016/j.cell.2015.06.013. 
            Epub 2015 Jul 2.
  PUBMED    26144318
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.06.013"; journalName: "Cell"; date: 
            "2015-07-16- 16"; volume: "162"; issue: "2"; pages: "300-13"
COMMENT     Sequence Label: pSR49
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             597..617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2284..2306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2406..2425
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2503..2521)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2589..2693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2694..2762,2763..3554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2912..2931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3725..4313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4214..4233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4467..4484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4601..4622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4637..4642,4643..4660,4661..4667)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4675..4691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4680..4702
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4699..4710,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4699..4710,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgct caattcagta agtacaactt taaaattggg
       61 ctgcaagatt tcttcttaaa ttttcaacag tttcaaaaca ctgaaatcat gctgaactat
      121 ggttagagac tcgacaactt tcctaaattt tggagcagtt tcaatggttt tcgaatgtat
      181 actacatttg taccatttta tgtgttagtg taggttgctt tgcttctacc ttcactctca
      241 aattcttttt tccgcggtaa tttttcaact atcgaaacaa agaagaagtc atgaataact
      301 gtccgcatag agttctcacc cgtccccttt cttttcaccg agcagtcacg agagaaagaa
      361 gaagaagcgg accgctgcag agattcttcg tagcggagcc ccgcccatcc tctgttacgc
      421 atatgtttgg atctcatttc ttccactcat taacgttcca ggtatgtttt ttttgttgat
      481 tttttaatgt gttacttttc atatgtttat tacttgaact gaccgatttc aaatatgatt
      541 ttcacaggca tgcctgcagg tcgactctag aggatccccg ggaaaaaatg actgctccaa
      601 agaagaagcg taaggtaaaa atgtccgaac tcatcaagga gaacatgcac atgaagctct
      661 acatggaggg aaccgtagac aaccaccact tcaagtgcac ctccgaggga gagggaaagc
      721 catacgaggg aacccaaacc atgcgtatca aggtcgtcga gggaggacca ctcccattcg
      781 ccttcgacat cctcgccacc tccttcctct acgggtccaa ggtaagttta aacatatata
      841 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc
      901 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg
      961 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct
     1021 acaacgtcaa gatccgtgga gtcaacttca cctccaacgg accagtcatg caaaagaaga
     1081 ccctcggatg ggagaccttc accgagaccc tctacccagc cgacggagga ctagagggac
     1141 gtaacgacat ggccctcaag ctcgtcggag gctcccacct catcgccaac gccaaggtaa
     1201 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagacc acctaccgtt
     1261 ccaagaagcc agccaagaac ctcaagatgc caggagtcta ctacgttgac taccgtctag
     1321 agcgtatcaa ggaggccaac aacgagacct acgtcgagca acacgaggtc gccgtcgccc
     1381 gttactgcga cctcccatcc aagctcggac acaagctcaa ctaagctagc gtcgacggta
     1441 ccatggtatt gatatctgag ctccgcatcg gccgctgtca tcagatcgcc atctcgcgcc
     1501 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     1561 tgctcccacc ccctattttt gttattatca aaaaaacttc ttcttaattt ctttgttttt
     1621 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     1681 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     1741 aaatctacac aatgttctgt gtacacttct tatgtttttt ttacttctga taaatttttt
     1801 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     1861 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     1921 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     1981 aattttcctg cttttgcttt ttgggggttt cccctattgt ttgtcaagag tttcgaggac
     2041 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     2101 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     2161 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     2221 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     2281 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     2341 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2401 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     2461 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     2521 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2641 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2701 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2761 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2821 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2881 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2941 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3001 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3061 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3121 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3181 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3241 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3301 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3361 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3421 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3481 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3541 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3601 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3661 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3721 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3781 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3841 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3901 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3961 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4081 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     4141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4261 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4321 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4381 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4441 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4501 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4561 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4621 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4681 gcggataaca atttcacaca ggaaacagct
//
