LOCUS       sequence-122071-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type PA-DAD in mammalian cells, an optogenetic tool 
            to activate endogenous diaphanous related formins in live cells 
            using light..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Rao MV, Chu PH, Hahn KM, Zaidel-Bar R
  TITLE     An optogenetic tool for the activation of endogenous 
            diaphanous-related formins induces thickening of stress fibers 
            without an increase in contractility.
  JOURNAL   Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 
            10.1002/cm.21115. Epub 2013 May 24.
  PUBMED    23677607
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.21115"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2013-07"; volume: 
            "70"; issue: "7"; pages: "394-407"
COMMENT     Sequence Label: PA-DAD-wt
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             597..629
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             636..659
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 agaagaactt tgtcattact gacccaagat tgccagataa tcccattata ttcgcgtccg
       61 atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga aactgcaggt
      121 ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat gccatagata
      181 accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa aagttctgga
      241 acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac tttattgggg
      301 ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga gtcatgctga
      361 ttaagaaaac tgcagaaaat atagatgagg cggcaaaaga aggtggaagt ggtggaagtg
      421 atgagacagg tgtgatggac agtcttctag aagctctgca gtcaggggca gcattccgac
      481 ggaagagagg gccccggcag gtcaacagga aggctgggtg tgcagtcaca tctctgctag
      541 cctcggagct gaccaagtaa aagcttgcgg ccgcacagct gtatacacgt gcaagccagc
      601 cagaactcgc cccggaagac cccgaggatc tcgagcacca ccatcaccat caccatcact
      661 aagtgattaa cctcaggtgc
//
