LOCUS       sequence-122074-        1347 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool
            to activate endogenous diaphanous related formins in live cells 
            using light with LOV2 in the closed conformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Rao MV, Chu PH, Hahn KM, Zaidel-Bar R
  TITLE     An optogenetic tool for the activation of endogenous 
            diaphanous-related formins induces thickening of stress fibers 
            without an increase in contractility.
  JOURNAL   Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 
            10.1002/cm.21115. Epub 2013 May 24.
  PUBMED    23677607
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.21115"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2013-07"; volume: 
            "70"; issue: "7"; pages: "394-407"
COMMENT     Sequence Label: PA-DAD-dark
FEATURES             Location/Qualifiers
     source          1..1347
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(31..33,34..744)
                     /codon_start=1
                     /product="photostable derivative of mVenus (Lee et al., 
                     2020)"
                     /label=mGold
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(334..353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             763..1188
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NMRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKE"
ORIGIN
        1 atggcacacc atcaccacca tcacggttct gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat cagcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcaa acttcaagat ccgccacaac
      541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagggttct ggaagtggat ccttggctac tacacttgaa
      781 cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc
      841 gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac
      901 atgaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc
      961 atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag
     1021 ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt
     1081 attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc
     1141 atgctgatta agaaaactgc agaaaatata gatgaggcgg caaaagaagg tggaagtggt
     1201 ggaagtgatg agacaggtgt gatggacagt cttctagaag ctctgcagtc aggggcagca
     1261 ttccgacgga agagagggcc ccggcaggtc aacaggaagg ctgggtgtgc agtcacatct
     1321 ctgctagcct cggagctgac caagtaa
//
