LOCUS       sequence-12208-e       10167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: Tailless VinculinTS
FEATURES             Location/Qualifiers
     source          1..10167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3553..4260
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4381..4383,4384..4386,4387..5097)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4426..4447)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4687..4706)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5034..5055
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5719..5737)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5720..5737)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5757..5774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5763..5987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6033..6461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6120..6139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6330..6351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6470..6490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6475..6805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6656..6791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6718..6737
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6872..7666
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6926..6945)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7536..7555
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7840..7961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7877..7896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7931..7950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8010..8026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8010..8026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8023..8045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8034..8050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8058..8064,8065..8082,8083..8088))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8103..8124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8241..8258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8412..9000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8492..8511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9171..9962,9963..10031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9794..9813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10032..10136)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccatg
      901 cccgtcttcc acacgcgcac catcgagagc atcttggagc ccgtggctca gcagatctcc
      961 cacctggtca tcatgcacga ggagggggag gtagacggca aggccatccc ggacctcacc
     1021 gcccccgtgt cggccgtgca ggccgctgtc agcaacctgg tgcgggttgg aaaagaaact
     1081 gtgcagacaa cagaagacca gatcttgaaa agggatatgc caccagcatt catcaaagta
     1141 gagaatgcct gcaccaagct cgttcgagca gcccagatgc tgcaagcaga tccttattca
     1201 gtaccagctc gtgactacct aattgatgga tcaagaggca tcctttctgg aacatcagac
     1261 ttgcttctga catttgatga agcagaggtc cgtaaaatca tccgtgtctg caaaggaata
     1321 ttggaatatc tgactgtggc agaagtagta gagactatgg aggatttggt gacatataca
     1381 aagaatctag ggccaggaat gacaaagatg gcaaaaatga tcgatgagag acaacaggaa
     1441 ttaactcatc aggaacatag ggttatgctg gtgaactcca tgaatactgt gaaggagcta
     1501 ttgcctgtac ttatttcagc tatgaagatc tttgtaacca caaaaaacac taaaagccag
     1561 ggaatagaag aggccttgaa aaatcgcaat ttcacagtag agaaaatgag tgctgagata
     1621 aatgaaataa tccgtgtatt acaactcact tcctgggatg aagatgcctg ggccagcaag
     1681 gacactgaag ccatgaaaag agctttagcc ctaatagatt caaagatgaa ccaggcaaaa
     1741 ggctggctga gagatccaaa cgcacctcca ggggatgctg gtgagcaagc aataaggcag
     1801 atccttgatg aagctggaaa agcaggagaa ttgtgtgcag gcaaagaacg cagggagatt
     1861 ctgggaacat gcaaaacttt gggccaaatg actgatcaac ttgctgatct ccgagctaga
     1921 ggacagggtg ctacaccgat ggcaatgcag aaggcacagc aggtgtcaca aggcctggat
     1981 ttgctcactg caaaagtgga gaatgcagcc cggaaattgg aggccatgac aaactctaag
     2041 caggctattg caaagaagat tgatgctgct cagaactggc ttgcggatcc caacggcgga
     2101 agtgaaggag aagaacacat tcgaggaatt atgtctgaag caaggaaagt tgcagaattg
     2161 tgtgaggagc ctaaagaaag agatgatatc cttcgctcct tgggggaaat ctctgctctg
     2221 acagctaagc tgtcagatct gcgacgacat gggaaaggcg actctcctga ggcccgtgca
     2281 ttggccaagc aaatagctac atcacttcag aacttacagt ccaaaacaaa cagagctgta
     2341 gcaaatacta ggccagttaa agctgctgtc catttggagg gcaagattga gcaagcccag
     2401 aggtggatag ataatcctac agttgatgat cggggagtag gccaggcagc aattcggggt
     2461 ttggttgcag aaggtcgtcg tctagccaat gtcatgatgg gaccttatcg tcaggacctg
     2521 cttgccaaat gtgaccgtgt agaccagctg gctgctcagc ttgctgacct tgcagcaaga
     2581 ggggaaggag agtctcctca ggctagggca attgctgctc agcttcagga ctccctgaag
     2641 gatctcaaag cacggatgca agaagcaatg acccaggagg tgtctgatgt ttttagtgac
     2701 actacaactc ctattaagtt gttagcagta gcagccactg ctccttctga tactcccaat
     2761 agagaagagg tgtttgaaga aagagcagca aattttgaaa accatgctgc tagactggga
     2821 gcaacagcag aaaaagcagc tgcagttgga actgctaata aaactactgt ggaaggcatt
     2881 caggcaacag tcaaatctgc aagggagctt acaccacagg tagtatcggc tgctcgaatc
     2941 ctcctgagaa atcctggaaa tcaagctgct tatgagcatt ttgagacaat gaaaaaccaa
     3001 tggattgata atgtagaaaa gatgacaggg ctggtggatg aggccatcga taccaagtct
     3061 ctgttggatg catcagaaga ggctattaag aaggatcttg ataaatgtaa agttgcaatg
     3121 gccaatatgc aacctcagat gctggtagct ggagccacca gcattgctag acgagcgaac
     3181 cgcatcctgc ttgtggcaaa acgggaggtt gaaaattcag aagaccctaa attcagggag
     3241 gctgttaaag cagcttctga tgagctgagc aaaaccatat caccgatggt aatggatgct
     3301 aaagctgtag caggaaatat ctctgatcct ggtttgcaga agagtttctt ggattctgga
     3361 tacaggattc tgggagctgt ggccaaagtc agagaagcct ttcagcctca ggagccagac
     3421 tttccccctc ctcctcctga cctcgagcat ctccatctga ctgatgagct tgctcctcca
     3481 aaaccaccac ttccagaagg tgaggttccc ccacccagac caccaccccc tgaagaaaaa
     3541 gatgaggtcg agatggtgag caagggcgag gagaccacaa tgggcgtaat caagcccgac
     3601 atgaagatca agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc
     3661 gagggcgagg gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga
     3721 gcccccctgc ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc
     3781 ttcaccaagt accccgacga catccccaac tacttcaagc agtccttccc cgagggctac
     3841 tcttgggagc gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc
     3901 tccatggagg aggactcctt catctacgag atacacctca agggcgagaa cttccccccc
     3961 aacggccccg tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac
     4021 gtgcgcgacg gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc
     4081 caccaccgcg ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac
     4141 tatcactttg tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg
     4201 accgtttacg agagcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtacaag
     4261 gggccaggtg gtgcagggcc aggtggtgca gggccaggtg gtgcagggcc aggtggtgca
     4321 gggcccggtg gtgcaggtcc aggtggtgca ggtccaggtg gtgcaggtcc aggtggtgct
     4381 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4441 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4501 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4561 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     4621 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4681 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4741 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4801 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     4861 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     4921 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4981 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5041 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggcg
     5101 gccgcggagt tcccagagca gaaagcagga gaagctatta atcagcccat gatgatggct
     5161 gctaggcagt tgcatgacga ggctcggaaa tggtctagca agggtaacga catcattgct
     5221 gctgctaaac gaatggcgct gctcatggct gagatgtcac gcctggtgcg aggaggcagc
     5281 ggaaacaagc gtgccctcat ccagtgtgca aaagatattg ctaaggcatc ggatgaagtc
     5341 actcgattgg ccaaagaggt ggccaagcaa tgtactgata aacgcattag aacaaacctc
     5401 ttacaggtct gtgagcgaat cccaaccatc agcacgcagc tcaaaattct ttccacagtg
     5461 aaagctacca tgctgggcag gactaacatc agcgatgaag aatcagaaca ggcaactgag
     5521 atgttggttc ataacgccca gaacctcatg cagtctgtga aggaaactgt gagagaagct
     5581 gaagcagcat ccattaagat aagaacagat gccggattca ctctgcgctg ggtcagaaag
     5641 accccatggt atcagtaaga attctgcaga tatccatcac actggcggcc gctcgagcat
     5701 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     5761 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5821 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5881 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5941 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6001 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6181 aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6241 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6361 caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg
     6421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6481 cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca
     6541 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6601 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6721 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6781 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     6841 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     6901 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     6961 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7021 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     7081 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7141 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7201 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7261 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7321 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7381 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7441 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7501 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7561 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7621 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7681 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     7741 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7801 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7861 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7921 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7981 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8221 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8581 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     8641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8821 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10141 tttccccgaa aagtgccacc tgacgtc
//
