LOCUS       sequence-122086-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags 
            in a mammalian system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, An
            J, Lamperti ED, Koh KP, Ganetzky R, Liu XS, Aravind L, Agarwal S, 
            Maciejewski JP, Rao A
  TITLE     Impaired hydroxylation of 5-methylcytosine in myeloid cancers with 
            mutant TET2.
  JOURNAL   Nature. 2010 Dec 9;468(7325):839-43. doi: 10.1038/nature09586.
  PUBMED    21057493
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-9- 9"; volume: "468"; issue: "7325"; pages:
            "839-43"
COMMENT     Sequence Label: FH-Tet2-pEF
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             142..165
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct
       61 aggtaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt cgcccttaga
      121 tcttccgctc gatgaaccat ggactacaag gacgacgatg acaagctcga tggaggatac
      181 ccctacgacg tgcccgacta cgccggagga ctcgaggaac aggacagaac cacccatgct
      241 gagggcacca gactgagtcc attcctgata gcaccacctt ctcccatcag ccatacagaa
      301 cctctggctg tcaaactcca gaatggaagc ccgttagcag agagacctca tccagaagta
      361 aatggagaca ccaagtggca atcttcccaa agctgttatg gaatatccca catgaaaggc
      421 agccagagca gtcatgagag tccacatgaa gacagagggt attccaggtg cttacagaat
      481 ggagggataa aacgcacagt cagtgaacca tctctctctg ggctccatcc aaacaagata
      541 ttgaaactag accaaaaagc taagggagaa agcaatatct tcgaggaaag ccaagaaaga
      601 aaccacggta aaagcagccg tcagccaaat gtctccggac taagtgataa tggagagccc
      661 gtgacctcga ccacccagga aagttcaggt gcagatgctt tcccaacacg gaactacaat
      721 ggagttgaaa ttcaggttct caacgagcag gaaggggaaa aaggcaggag cgttacatta
      781 cttaaaaaca agattgtgct aatgcctaat ggtgctacag ttt
//
