LOCUS       sequence-122089-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K3.loxP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..209)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(248..445)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
       61 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      121 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcggtctc tactgataac
      181 ttcgtatagc atacattata cgaagttatt gctagcctgg atggcggcgt tagtatcgaa
      241 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
      301 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
      361 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
      421 aaacaagaat ctttttattg tcagtactct ttacaacact cccttcgtgc ttgggacttc
      481 agaacttcca gtaagactag tagccgcgcg catggcaaca gccagagatt taaaagcgct
      541 ttcagcacga tgatggtcat tactaccata taagcaggta acatgcaaag taattccagc
      601 tgctaccgaa aaggaatata gtaagtgagg gatcatttca caggacaatt ccccaacctt
      661 ttcacgcttt aatcccaaat cgataacagc atagggccgt cccgacaagt caactacgct
      721 tctagaaagg gcttcgtcaa gtggacaata agcatgtcca aatcttttaa cgccggcaaa
      781 gttacccata gcctgcttga atgcaatacc aagtgcaata gcagtatctt ctgcagtgtg
      841 atgatcatcg atgattaaat cacctcttga gtaaagtcgt aagctc
//
