LOCUS       sequence-122090-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K3.loxP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             173..184
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(226..232,233..250,251..256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    271..292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    404..437
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acggcagcgg accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac
       61 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa
      121 ctttgaaacg catgaactct ttgataacgt cttcggagga agccatctag tatttctcct
      181 ctttctctag tatgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      361 ccaacgcgcg gggagaggcg gtttgcgtat tgggtctcat tttataactt cgtataatgt
      421 atgctatacg aagttatact agtgcatgct ttggacagtg ataccgaaga aggaagtaaa
      481 ttcagtagcg ccgcatgagg tagtagcaga aggagttaag gttgaccatg gctcacttgg
      541 catatactgt tgggcagaaa cagtatagtg aaactgttat caaaccaata tatttgggct
      601 acatggcttt cctttcccga aaaagacacc atttagtaat tatgttgtac ttttactctc
      661 tgaacttctg tgaatataaa aaatatacta aataaaagga tgcaacttcc ttaaagaggg
      721 caagatagcg acataaagtg gaaaagtgtt agcaacattg cgcaggcatc gattaatata
      781 tgcatatata ctttttacgt agtctacgtt aaattgtcaa
//
