LOCUS       sequence-122092-        7952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A 
            and L567A substitutions) tagged with EGFP along with shRNA to knock 
            down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7952)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 7952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP(L566,567A)-shTACC3
FEATURES             Location/Qualifiers
     source          1..7952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..86
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     78..95
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     79..95
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     102..121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        102..120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     153..169
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        189..403
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     324..343
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(476..492)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(477..493)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(521..539)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(560..576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(573..595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(608..614,615..632,633..638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    653..674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     enhancer        777..1080
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1081..1284
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1235..1255
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1329..1331,1332..1334,1335..2045)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1329..1331,1332..1334,1335..2045)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1374..1395)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1635..1654)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1982..2003
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4740..4861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4777..4796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4831..4850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4868..5323)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5005..5026)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5217..5236
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5350..5454
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5456..5813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5456..5647
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5479..5499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5664..5799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5848..6642
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5848..6642
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5902..5921)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6512..6531
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6830..6849)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6874..6921
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7739..7758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
       61 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
      121 gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc
      181 aggaattcga acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta
      241 ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag
      301 tggcgccctg caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa
      361 tgtctttgga tttgggaatc ttataagttc tgtatgagac cacagatccc cgttaccgga
      421 agatcgtctg ttcaagagac agacgatctt ccggtaactt tttggaaaag cttatcgata
      481 ccgtcgacct cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt
      541 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      721 gccagatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      781 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      841 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      901 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      961 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1021 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1081 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     1141 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     1201 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     1261 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     1321 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1381 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1441 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1501 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1561 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1621 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1681 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1741 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1801 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1861 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1921 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1981 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2041 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
     2101 gcccgggtat gagtctgcag gtcttaaacg acaaaaatgt cagcaatgaa aaaaatacag
     2161 aaaattgcga cttcctgttt tcgccaccag aagttacagg taggtcatct gttcttcgtg
     2221 tgtcacagaa agaaaatgtg ccacccaaga acctggccaa agctatgaag gtgacttttc
     2281 agacacctct gcgggatcca cagacgcaca ggattctaag tcctagcatg gccagcaaac
     2341 ttgaggctcc tttcactcag gatgacaccc ttggactgga aaactcacac ccggtctgga
     2401 cacagaaaga gaaccaacag ctcatcaagg aagtggatgc caaaactact catggaattc
     2461 tacagaaacc agtggaggct gacaccgacc tcctggggga tgcaagccca gcctttggga
     2521 gtggcagctc cagcgagtct ggcccaggtg ccctggctga cctggactgc tcaagctctt
     2581 cccagagccc aggaagttct gagaaccaaa tggtgtctcc aggaaaagtg tctggcagcc
     2641 ctgagcaagc cgtggaggaa aaccttagtt cctattcctt agacagaaga gtgacacccg
     2701 cctctgagac cctagaagac ccttgcagga cagagtccca gcacaaagcg gagactccgc
     2761 acggagccga ggaagaatgc aaagcggaga ctccgcacgg agccgaggag gaatgccggc
     2821 acggtggggt ctgtgctccc gcagcagtgg ccacttcgcc tcctggtgca atccctaagg
     2881 aagcctgcgg aggagcaccc ctgcagggtc tgcctggcga agccctgggc tgccctgcgg
     2941 gtgtgggcac ccccgtgcca gcagatggca ctcagaccct tacctgtgca cacacctctg
     3001 ctcctgagag cacagcccca accaaccacc tggtggctgg cagggccatg accctgagtc
     3061 ctcaggaaga agtggctgca ggccaaatgg ccagctcctc gaggagcgga cctgtaaaac
     3121 tagaatttga tgtatctgat ggcgccacca gcaaaagggc acccccacca aggagactgg
     3181 gagagaggtc cggcctcaag cctcccttga ggaaagcagc agtgaggcag caaaaggccc
     3241 cgcaggaggt ggaggaggac gacggtagga gcggagcagg agaggacccc cccatgccag
     3301 cttctcgggg ctcttaccac ctcgactggg acaaaatgga tgacccaaac ttcatcccgt
     3361 tcggaggtga caccaagtct ggttgcagtg aggcccagcc cccagaaagc cctgagacca
     3421 ggctgggcca gccagcggct gaacagttgc atgctgggcc tgccacggag gagccaggtc
     3481 cctgtctgag ccagcagctg cattcagcct cagcggagga cacgcctgtg gtgcagttgg
     3541 cagccgagac cccaacagca gagagcaagg agagagcctt gaactctgcc agcacctcgc
     3601 ttcccacaag ctgtccaggc agtgagccag tgcccaccca tcagcagggg cagcctgcct
     3661 tggagctgaa agaggagagc ttcagagacc ccgctgaggt tctaggcacg ggcgcggagg
     3721 tggattacct ggagcagttt ggaacttcct cgtttaagga gtcggccttg aggaagcagt
     3781 ccttatacct caagttcgac cccgccgcga gggacagtcc tggtagacca gtgcccgtgg
     3841 ccaccgagac cagcagcatg cacggtgcaa atgagactcc ctcaggacgt ccgcgggaag
     3901 ccaagcttgt ggagttcgat ttcttgggag cactggacat tcctgtgcca ggcccacccc
     3961 caggtgttcc cgcgcctggg ggcccacccc tgtccaccgg acctatagtg gacctgctcc
     4021 agtacagcca gaaggacctg gatgcagtgg taaaggcgac acaggaggag aaccgggagc
     4081 tgaggagcag gtgtgaggag ctccacggga agaacctgga actggggaag atcatggaca
     4141 ggttcgaaga ggttgtgtac caggccatgg aggaagttca gaagcagaag gaactttcca
     4201 aagctgaaat ccagaaagtt ctaaaagaaa aagaccaact taccacagat ctgaactcca
     4261 tggagaagtc cttctccgac ctcttcaagc gttttgagaa acagaaagag gtgatcgagg
     4321 gctaccgcaa gaacgaagag tcactgaaga agtgcgtgga ggattacctg gcaaggatca
     4381 cccaggaggg ccagaggtac caagccctga aggcccacgc ggaggagaag ctgcagctgg
     4441 caaacgagga gatcgcccag gtccggagca aggcccaggc ggaagcgttg gccctccagg
     4501 ccagcctgag gaaggagcag atgcgcatcc agtcgctgga gaagacagtg gagcagaaga
     4561 ctaaagagaa cgaggagctg accaggatct gcgacgacct catctccaag atggagaaga
     4621 tctctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4681 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4861 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4921 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4981 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5041 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5101 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5161 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5221 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5281 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     5461 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     5821 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5881 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5941 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6001 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     6061 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6121 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6181 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6241 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6301 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6361 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6421 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6481 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6541 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6601 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6661 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6721 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6781 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     6841 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     6901 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     6961 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     7021 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     7081 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7921 attctgtgga taaccgtatt accgccatgc at
//
