LOCUS       sequence-122101-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the generation of gene disruptions via Bxb1-mediated 
            recombination into MIN-tagged cell lines. This vector can also be 
            used to express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Poly(A)
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..84
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             87..791
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(229..247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     497..516
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    899..1033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(923..942)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     977..996
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct agtgtgcgga tatcccggct tgtcgacgac
       61 ggcggtctcc gtcgtcagga tcatccgtga gcaagggcga ggaggataac atggccatca
      121 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      301 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      421 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      481 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      541 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      601 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      661 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      721 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      781 agctgtacaa gagcggcctg aggagcagag cccaggcgag caacagcgcc gtggacgcca
      841 ccatgggcga tcgcccggga attgactagt gcggccgcag ttaacgaatt ctctagagga
      901 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      961 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1021 caataaacaa gttaacaaca acaattgcat tcatttt
//
