LOCUS       sequence-12212-e        2858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, 
            Gerhardt H, Alitalo K, Lawson ND
  TITLE     A truncation allele in vascular endothelial growth factor c reveals 
            distinct modes of signaling during lymphatic and vascular 
            development.
  JOURNAL   Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub
            2013 Mar 5.
  PUBMED    23462469
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.084152"; journalName: "Development"; date: "2013-04"; 
            volume: "140"; issue: "7"; pages: "1497-506"
COMMENT     Sequence Label: 475 p5EfliEBSbasP
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    904..1027
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1099..1118)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1100..1118)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1123..1139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1123..1139)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1241..1260)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1252..2061
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1319..1338)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2208..2796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2697..2716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa cttgtataga aaagttgtac acactttagt cgacaatgag aagggtgtct
      721 caatctatgg gtatttcctg ttccacacaa tctggtttcc tgtcaggttt ccttttcctc
      781 ctgaaacaaa agacaggaat gccctctgta taaaatagca cagcgctttt aatgggtctc
      841 tgtgtactag agggtatata atggatcatc gcgtctcagc ctcactttga gctcctccac
      901 acgcaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat
      961 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt
     1021 cactatgaat caactactta gatggtatta gtgacctgta gaattcgagc tctagagctg
     1081 cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1141 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1201 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1261 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1321 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1381 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1441 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1501 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1561 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1621 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1681 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1741 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1801 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1861 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1921 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1981 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2041 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2101 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2341 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2581 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2821 cggttcctgg ccttttgctg gccttttgct cacatgtt
//
