LOCUS       sequence-122148-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-lacZYA
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     969..991
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1016..1032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1016..1032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1029..1051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1040..1056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attttcaatc cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc
       61 gtcggcggtg tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag
      121 tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg gcataaccac cacgctcatc
      181 gataatttca ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa
      241 agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag
      301 tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt
      361 agtcggttta tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg
      421 atcttccaga taactgccgt cactccagcg cagcaccatc accgcgaggc ggttttctcc
      481 ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa cgactgtcct ggccgtaacc
      541 gacccagcgc ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg
      601 cgtctggcct tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt
      661 cggattctcc gtgggaacaa acggcggatt gaccgtaatg ggataggtca cgttggtgta
      721 gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc
      781 aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa
      841 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      901 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      961 agggttttcc cagtcacgac gttgtaaaac gacgggatcg caagcttgaa tcatggtcat
     1021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattct gcaca
//
