LOCUS       sequence-122151-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens 
            (B. japonicum) scoI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ledermann R, Bartsch I, Remus-Emsermann M, Vorholt JA, Fischer HM
  TITLE     Stable fluorescent and enzymatic tagging of Bradyrhizobium 
            diazoefficiens to analyze host-plant infection and colonization.
  JOURNAL   Mol Plant Microbe Interact. 2015 Jun 2.
  PUBMED    26035130
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2015 Jun 2."
COMMENT     Sequence Label: pRJPaph-lacZYA
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    447..463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     452..474
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     471..487
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     471..487
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(512..534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
       61 tcaccgtctt tcattgccat acggaattcg agctcgcacg ctgccgcaag cactcagggc
      121 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
      181 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
      241 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
      301 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
      361 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc
      421 aagagacagg atactagtgt gcagaattgt gagcggataa caatttcaca caggaaacag
      481 ctatgaccat gattcaagct tgcgatcccg tcgttttaca acgtcgtgac tgggaaaacc
      541 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      601 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      661 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
      721 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
      781 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
      841 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
      901 cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg
      961 tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttt
//
