LOCUS       sequence-122164-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express soluble His-tagged zebrafish thrombopoietin in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Svoboda O, Stachura DL, Machonova O, Pajer P, Brynda J, Zon LI, 
            Traver D, Bartunek P
  TITLE     Dissection of vertebrate hematopoiesis using zebrafish 
            thrombopoietin.
  JOURNAL   Blood. 2014 Jul 10;124(2):220-8. doi: 10.1182/blood-2014-03-564682. 
            Epub 2014 May 28.
  PUBMED    24869937
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2014-03-564682"; journalName: "Blood"; date: 
            "2014-07-10- 10"; volume: "124"; issue: "2"; pages: "220-8"
COMMENT     Sequence Label: pAc-­His-­zfTpo
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(961..978)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacagcttca tcgtgtcggg gtttaacatt acggatttcc ttgaagagag tgagtttttg
       61 gttcttgccg ggtcccagga aaggatcaga tctgcagcgg ccgctccgga attcccatgg
      121 cccgggatca gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
      181 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
      241 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg
      301 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
      361 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
      421 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc
      481 ctagcaccat tttgccatct ctgcaatgag caactctagc ttgcctgtga gcagaagccc
      541 aaagtacttc agcaccaaac ccaaatcctt cgagggctga cccagacacc tctctaccgc
      601 tggcccttcc tgctccccct gctccacgtg gagcagtctg actacatgca ggtagttgtt
      661 gatactgtgt tccaagcgct tcagcagagt gagtccacag ccaggttggc tgagtgtcct
      721 ggcagaccga acatcctgcg ctagagtccc caaagacagc aggatctcgg ccctgcgctc
      781 ctgtactgat tttctctccc aggttgcttt gtgaatgctg atgcatggta attgaataga
      841 agaggggaga gcgtctacgc cactgcagat taccttttct tcttgtagat ccttcacctt
      901 gttcatcact ctcctggcct cgctgtcaca cacaaagtca attggtctag tggatccacc
      961 atggtgatgg tgatggtggc tagccataga tccaagatcc gccgcaaagg cagaatgcgc
     1021 cgccgccgcc aaagcacata taaaacaata gcgcttacca tcttgct
//
