LOCUS       sequence-122165-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a HdV/M-domain 
            3'-tag for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Kieft JS, Batey RT
  TITLE     A general method for rapid and nondenaturing purification of RNAs.
  JOURNAL   RNA. 2004 Jun;10(6):988-95.
  PUBMED    15146082
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-06"; volume: "10(6)"; pages: "988-95"
COMMENT     Sequence Label: pRAV12
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(259..278)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(260..278)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(285..302)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(285..301)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(294..316)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(510..529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     629..651
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(689..707)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        775..879
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccctcaagac ccgtttagag gccccaaggg gttatgctag ggatcctgcc cagggctgct
       61 acccgaaggt cctgacccga tgcacaggaa aaaactgccc agggctgcta cctttcagtc
      121 ctgacccgat gcacagtcta gaggtcccat tcaccatgcc gaagcatgtt gcccaccggc
      181 cgccagcgag gaggctggga ccatggccgg cttgtcttcg agattctttt cgcacaacac
      241 cacttaatgt ttgagcgacc ctatagtgag tcgtattaga attcactggc cgtcgtttta
      301 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      361 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      421 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      481 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      541 cagccccgac acccgccaac acccactgac gcgccctgac gggcttgtct gctcccggca
      601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      901 gtcgccctta ttcccttttt t
//
