LOCUS       sequence-122179-       13654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB329
FEATURES             Location/Qualifiers
     source          1..13654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..3501
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      3561..3813
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3847..3863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3847..3863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3860..3882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3871..3887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3895..3901,3902..3919,3920..3925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3940..3961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4046..4221
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6305..6981)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6348..6368)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7176..7200
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7721..8512
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8758..9346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9247..9266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9500..9517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9532..9672
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9598..9617)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9758..9780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10016..10210
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10276..11349)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11778..12407)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ctggaggctc cggtgggagc atggtgagca agggcgagga ggtcatcaaa
     2101 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     2161 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2221 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     2281 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     2341 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     2401 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     2461 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2521 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     2581 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     2641 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     2701 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
     2761 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
     2821 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     2881 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2941 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     3001 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     3061 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     3121 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     3181 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     3241 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     3301 aaggacggcg gccgctacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     3361 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     3421 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     3481 atggacgagc tgtacaagta agcttgcatg cctgcaggtc gactctagag gatccccggg
     3541 taccgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     3601 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     3661 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     3721 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     3781 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     3841 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3901 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3961 aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt ttaatgtact
     4021 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga
     4081 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
     4141 tatgctcaac acatgagcga aaccctatag gaaccctaaa ttcccttatc tgggaactac
     4201 tcacacatta ttatggagaa actcgaagtt agtatatagt ccttccatca ccctctgtga
     4261 taatctcctt aaaacttgct cccccaggaa tcataggcag aacgtgctcc tggtggggaa
     4321 ctatcacatc cagtaaataa ggaccgggag aatctagcat agtttgaatt gctgctctta
     4381 gttcatcctt cttcaccaca cgcgcatttg gcaccttgaa accgtctgca actgtacaga
     4441 agtcggggta gatctgctcc tcgtgatctg gatcacctaa gtaagtatgt gctctgttgg
     4501 ccttgtagaa tctatcctcc aattgtacga ccattcctag atgttggttg tttaagatca
     4561 tgatcttcac gggtaacttc tccacaaaaa ttgtagccaa ctcctgtacg ttcatcacaa
     4621 agctaccatc gccatcgatg tcgatcacag gaatatctgg ttgagttgcg gcagccccca
     4681 aagcagaagg aagtccgaat cccatggctc caagacctcc agatgtcaac caacgccgcg
     4741 gttcgttgta cttgaaccac tgggctgccc acatttgatg ttggccaaca ccagtagata
     4801 tgatggcatt tccaccagtg agttcgtaca gcgtctgtat ggcagattga ggagcaatgg
     4861 catcctcgaa tacgggaaac ttcattggcc atttctgctt ttgttcttct agttctgcca
     4921 cccagttgga gaagtcgaaa gtcctctttt gactcttcaa gagggcattg atttcactta
     4981 gcgcaggttt cacatcagca cacatggaca catggggctg tttgttcttt ccaatttcag
     5041 cagggtcaat atcgatatga acgatgctgg acctgctagc aaaagtctcc agtttaccag
     5101 tcactcgatc gtcgaaacgc actccaaatg ccaacaacag atctgcttgg tccacggcgt
     5161 agttggcgta aacagttcca tgcattccaa gcattttcaa acttaatgga tcagactcgg
     5221 gatacgctcc caaacccatt aaagtcgatg tgataggaat tccggtaagc tcgacaaatt
     5281 ccctcaactc ttcgctggcg tgattgcaac ctcctccaga atacactaca ggcttcttag
     5341 aactagaaat gagtcttata atctgctcga ttaacgttgg ttccggtggc cgaggcagtc
     5401 gttgtaagta gcccttcagt tgcattggct gagaccaatc tggaatagcc atttgctgtt
     5461 gcacgtcctt gggaatatct accaagactg ggcccggcct acctgaagct gccaaaaaga
     5521 aagcctccct gattacgcga gggatgtcat caatgttcat aacaaggtaa ttgtgcttgg
     5581 tgatcgatct cgtaacctcc acaattggag tttcttgaaa agcatcggta ccaatcatcc
     5641 tactggatac ttgacctgta atggccacta gtggcacact gtccaagagg gcatcagcga
     5701 gcccggtaac aagattggtc gctccaggac cggatgtggc tatacaaacc cctactcgtc
     5761 cagacgattt agcataacct tcagcagcga aaatctcccc ctgctcgtgg cgacagagaa
     5821 tgttgcggat gctgctggaa cgtgtaagag cctggtgaat ctccatagac gctccgccgg
     5881 gataagcgaa caccgttcta atcccttctc gctcaagagc ctccaccaaa atgtcggctc
     5941 ctttgcgggg ctcgtcatcc ttgtagcgag actgaaagtc ggacggggtt ggtgcaacga
     6001 gtgacttccc gctgctaagc cgtggtccac tcacatcgac tgcagactgg atcttctcgc
     6061 taacagcttg ggctttcact tccagtgaac gctcagtctt ccgagaggtg ttgtatgcat
     6121 gtcccctcag agcggctccc gagaagaaac cactcctcac ctttagctct ttagctccct
     6181 tgcaacaaga ccttgccgat ggctgcacct tctccaggct cgctatgctc aaaggcacag
     6241 ctgcgcatcc actaattgac gccatgctcg agagagatag atttgtagag agagactggt
     6301 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     6361 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     6421 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     6481 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     6541 tatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     6601 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     6661 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     6721 ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     6781 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     6841 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     6901 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     6961 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     7021 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
     7081 aaccgaattc agaaataaat tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc
     7141 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     7201 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     7261 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     7321 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     7381 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     7441 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     7501 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     7561 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     7621 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     7681 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     7741 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     7801 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     7861 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     7921 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     7981 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     8041 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     8101 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     8161 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     8221 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     8281 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     8341 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     8401 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     8461 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     8521 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     8581 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     8641 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     8701 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8761 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8881 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9421 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9481 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     9541 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     9601 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     9661 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9781 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     9841 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     9901 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     9961 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
    10021 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
    10081 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
    10141 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
    10201 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
    10261 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
    10321 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
    10381 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
    10441 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
    10501 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
    10561 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
    10621 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
    10681 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
    10741 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
    10801 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
    10861 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
    10921 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
    10981 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
    11041 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
    11101 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
    11161 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
    11221 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
    11281 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    11341 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    11401 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    11461 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    11521 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    11581 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    11641 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    11701 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    11761 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    11821 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    11881 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    11941 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    12001 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    12061 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    12121 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    12181 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    12241 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    12301 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    12361 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    12421 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    12481 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    12541 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    12601 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    12661 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    12721 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    12781 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    12841 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    12901 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    12961 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    13021 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    13081 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    13141 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    13201 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    13261 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    13321 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    13381 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    13441 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    13501 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    13561 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
    13621 gcatgcacat acaaatggac gaacggataa acct
//
