LOCUS       sequence-122181-       13557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB130
FEATURES             Location/Qualifiers
     source          1..13557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2927..4357
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      4417..4669
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4703..4719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4703..4719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4716..4738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4727..4743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4751..4757,4758..4775,4776..4781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4796..4817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4902..5076
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5117..6142)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6209..6884)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    7079..7103
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7624..8415
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8661..9249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9150..9169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9403..9420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9435..9575
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9501..9520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9661..9683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9919..10113
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10179..11252)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11681..12310)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatgg tgagcaaggg
     2941 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     3001 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3061 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     3121 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     3181 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     3241 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     3301 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3361 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     3421 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     3481 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     3541 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     3601 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     3661 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     3721 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3781 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     3841 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     3901 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3961 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     4021 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     4081 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     4141 ccaggccctg aagctgaagg acggcggccg ctacctggtg gagttcaaga ccatctacat
     4201 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     4261 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     4321 cctgttcctg tacggcatgg acgagctgta caagtaagct tgcatgcctg caggtcgact
     4381 ctagaggatc cccgggtacc gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     4441 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     4501 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     4561 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     4621 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     4681 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4741 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4801 tgagctaact cacattaggc tgaattaggc gcgcctattt ctgaattcaa cacattgcgg
     4861 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg
     4921 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
     4981 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc
     5041 ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga tcgacagatc
     5101 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
     5161 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     5221 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     5281 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     5341 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     5401 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     5461 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     5521 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     5581 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     5641 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     5701 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     5761 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
     5821 gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag atgcaatagg
     5881 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     5941 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     6001 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     6061 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     6121 cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag ctcgagagag
     6181 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
     6241 cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
     6301 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     6361 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     6421 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     6481 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     6541 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     6601 ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg
     6661 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     6721 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     6781 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     6841 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     6901 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg
     6961 caagctgctc tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt
     7021 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
     7081 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
     7141 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
     7201 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
     7261 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
     7321 gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt
     7381 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     7441 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
     7501 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     7561 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     7621 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     7681 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     7741 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     7801 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     7861 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     7921 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     7981 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     8041 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     8101 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     8161 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     8221 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     8281 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     8341 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     8401 gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg
     8461 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc
     8521 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata
     8581 tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9421 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9481 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     9541 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     9601 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     9661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
     9721 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
     9781 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
     9841 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
     9901 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
     9961 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    10021 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    10081 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    10141 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    10201 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    10261 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    10321 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    10381 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    10441 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    10501 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    10561 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    10621 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    10681 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    10741 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    10801 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    10861 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    10921 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    10981 tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg
    11041 ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt
    11101 cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc
    11161 tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt
    11221 tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag
    11281 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc
    11341 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc
    11401 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc
    11461 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc
    11521 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct
    11581 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc
    11641 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa
    11701 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa
    11761 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa
    11821 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt
    11881 tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct
    11941 gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg
    12001 gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg
    12061 ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg
    12121 ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg
    12181 cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc
    12241 gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga
    12301 taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc
    12361 gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc
    12421 ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc
    12481 caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc
    12541 cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg
    12601 gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc
    12661 tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc
    12721 tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc
    12781 tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg
    12841 gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc
    12901 cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac
    12961 accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc
    13021 gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg
    13081 cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc
    13141 agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg
    13201 gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca
    13261 tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg
    13321 caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg
    13381 gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc
    13441 actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga
    13501 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
