LOCUS       sequence-122184-       14510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB330
FEATURES             Location/Qualifiers
     source          1..14510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2083
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..2730
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2609..2628
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2927..4357
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     terminator      4417..4669
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4703..4719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4703..4719)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4716..4738)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4727..4743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4751..4757,4758..4775,4776..4781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4796..4817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4902..5077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7161..7837)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    8032..8056
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8577..9368
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9614..10202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10103..10122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10356..10373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10388..10528
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10454..10473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10614..10636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10872..11066
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11132..12205)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12634..13263)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgctgg aggctccggt gggagcatgg tgagcaaggg
     2941 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     3001 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3061 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     3121 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     3181 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     3241 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     3301 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3361 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     3421 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     3481 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     3541 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     3601 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     3661 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     3721 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3781 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     3841 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     3901 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3961 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     4021 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     4081 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     4141 ccaggccctg aagctgaagg acggcggccg ctacctggtg gagttcaaga ccatctacat
     4201 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     4261 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     4321 cctgttcctg tacggcatgg acgagctgta caagtaagct tgcatgcctg caggtcgact
     4381 ctagaggatc cccgggtacc gagctcgaat ttccccgatc gttcaaacat ttggcaataa
     4441 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     4501 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     4561 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     4621 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt
     4681 ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4741 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4801 tgagctaact cacattaggc tgaattaggc gcgcctattt ctgaattcaa cacattgcgg
     4861 acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg
     4921 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
     4981 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaaattcc
     5041 cttatctggg aactactcac acattattat ggagaaactc gaagttagta tatagtcctt
     5101 ccatcaccct ctgtgataat ctccttaaaa cttgctcccc caggaatcat aggcagaacg
     5161 tgctcctggt ggggaactat cacatccagt aaataaggac cgggagaatc tagcatagtt
     5221 tgaattgctg ctcttagttc atccttcttc accacacgcg catttggcac cttgaaaccg
     5281 tctgcaactg tacagaagtc ggggtagatc tgctcctcgt gatctggatc acctaagtaa
     5341 gtatgtgctc tgttggcctt gtagaatcta tcctccaatt gtacgaccat tcctagatgt
     5401 tggttgttta agatcatgat cttcacgggt aacttctcca caaaaattgt agccaactcc
     5461 tgtacgttca tcacaaagct accatcgcca tcgatgtcga tcacaggaat atctggttga
     5521 gttgcggcag cccccaaagc agaaggaagt ccgaatccca tggctccaag acctccagat
     5581 gtcaaccaac gccgcggttc gttgtacttg aaccactggg ctgcccacat ttgatgttgg
     5641 ccaacaccag tagatatgat ggcatttcca ccagtgagtt cgtacagcgt ctgtatggca
     5701 gattgaggag caatggcatc ctcgaatacg ggaaacttca ttggccattt ctgcttttgt
     5761 tcttctagtt ctgccaccca gttggagaag tcgaaagtcc tcttttgact cttcaagagg
     5821 gcattgattt cacttagcgc aggtttcaca tcagcacaca tggacacatg gggctgtttg
     5881 ttctttccaa tttcagcagg gtcaatatcg atatgaacga tgctggacct gctagcaaaa
     5941 gtctccagtt taccagtcac tcgatcgtcg aaacgcactc caaatgccaa caacagatct
     6001 gcttggtcca cggcgtagtt ggcgtaaaca gttccatgca ttccaagcat tttcaaactt
     6061 aatggatcag actcgggata cgctcccaaa cccattaaag tcgatgtgat aggaattccg
     6121 gtaagctcga caaattccct caactcttcg ctggcgtgat tgcaacctcc tccagaatac
     6181 actacaggct tcttagaact agaaatgagt cttataatct gctcgattaa cgttggttcc
     6241 ggtggccgag gcagtcgttg taagtagccc ttcagttgca ttggctgaga ccaatctgga
     6301 atagccattt gctgttgcac gtccttggga atatctacca agactgggcc cggcctacct
     6361 gaagctgcca aaaagaaagc ctccctgatt acgcgaggga tgtcatcaat gttcataaca
     6421 aggtaattgt gcttggtgat cgatctcgta acctccacaa ttggagtttc ttgaaaagca
     6481 tcggtaccaa tcatcctact ggatacttga cctgtaatgg ccactagtgg cacactgtcc
     6541 aagagggcat cagcgagccc ggtaacaaga ttggtcgctc caggaccgga tgtggctata
     6601 caaaccccta ctcgtccaga cgatttagca taaccttcag cagcgaaaat ctccccctgc
     6661 tcgtggcgac agagaatgtt gcggatgctg ctggaacgtg taagagcctg gtgaatctcc
     6721 atagacgctc cgccgggata agcgaacacc gttctaatcc cttctcgctc aagagcctcc
     6781 accaaaatgt cggctccttt gcggggctcg tcatccttgt agcgagactg aaagtcggac
     6841 ggggttggtg caacgagtga cttcccgctg ctaagccgtg gtccactcac atcgactgca
     6901 gactggatct tctcgctaac agcttgggct ttcacttcca gtgaacgctc agtcttccga
     6961 gaggtgttgt atgcatgtcc cctcagagcg gctcccgaga agaaaccact cctcaccttt
     7021 agctctttag ctcccttgca acaagacctt gccgatggct gcaccttctc caggctcgct
     7081 atgctcaaag gcacagctgc gcatccacta attgacgcca tgctcgagag agatagattt
     7141 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
     7201 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
     7261 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     7321 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     7381 tttcctttat cgcaattatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     7441 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     7501 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     7561 acgagagtgt cgtgctccac catgttcaca tcaatccact tgctttgaag acgtggttgg
     7621 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     7681 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     7741 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     7801 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     7861 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
     7921 ctctagccaa tacgcaaacc gaattcagaa ataaattcag cctaattcgg cgttaattca
     7981 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     8041 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     8101 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     8161 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     8221 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     8281 gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     8341 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     8401 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     8461 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     8521 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg
     8581 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     8641 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     8701 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     8761 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     8821 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     8881 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     8941 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     9001 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     9061 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     9121 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     9181 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     9241 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     9301 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     9361 gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac
     9421 tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg
     9481 cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca
     9541 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9601 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9661 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9721 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     9781 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9841 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9901 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9961 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10021 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10081 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10141 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10201 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10261 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    10321 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10381 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    10441 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
    10501 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
    10561 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    10621 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
    10681 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
    10741 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
    10801 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
    10861 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
    10921 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
    10981 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
    11041 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
    11101 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
    11161 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
    11221 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
    11281 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
    11341 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    11401 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    11461 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    11521 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    11581 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    11641 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    11701 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    11761 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    11821 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    11881 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    11941 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    12001 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    12061 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    12121 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    12181 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    12241 gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    12301 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    12361 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    12421 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    12481 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    12541 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    12601 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    12661 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    12721 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    12781 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    12841 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    12901 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    12961 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    13021 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    13081 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    13141 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    13201 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    13261 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    13321 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    13381 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    13441 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    13501 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    13561 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    13621 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    13681 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    13741 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    13801 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    13861 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    13921 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    13981 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    14041 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    14101 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    14161 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    14221 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    14281 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    14341 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    14401 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    14461 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
