LOCUS       sequence-122189-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugally transferable Flp recombinase plasmid for unmarked 
            mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Hossain MJ, Thurlow CM, Sun D, Nasrin S, Liles MR
  TITLE     Genome modifications and cloning using a conjugally transferable 
            recombineering system.
  JOURNAL   Biotechnol Rep (Amst). 2015 Aug 28;8:24-35. doi: 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec.
  PUBMED    28352570
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Rep (Amst)."; date: "2015-08-28"; volume: "8:24-35. doi"; pages: " 
            10.1016/j.btre.2015.08.005. eCollection 2015 Dec"
COMMENT     Sequence Label: pCMT-flp
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(359..364,365..381,382..387))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             511..798
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated and modified)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /note="modified to prevent cleavage by eukaryotic SUMO 
                     proteases"
                     /translation="DSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRR
                     LMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
       61 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
      121 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
      181 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
      241 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
      301 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
      361 taaagcttat cgatgataag ctgtcaaaca tgagaattac aacttatatc gtatggggct
      421 gacttcaggt gctacactag aaataatttt gtttaactat aagaaggaga tatacatatg
      481 catcatcacc accatcacgg gtccctgcag gactcagaag tcaatcaaga agctaagcca
      541 gaggtcaagc cagaagtcaa gcctgagact cacatcaatt taaaggtgtc cgatggatct
      601 tcagagatct tcttcaagat caaaaagacc actcctttaa gaaggctgat ggaagcgttc
      661 gctaaaagac agggtaagga aatggactcc ttaacgttct tgtacgacgg tattgaaatt
      721 caagctgatc agacccctga agatttggac atggaggata acgatattat tgaggctcac
      781 cgcgaacaga ttggaggtcc acaatttggt atattatgta aaacaccacc taaggtgctt
      841 gttcgtcagt ttgtggaa
//
