LOCUS       sequence-122191-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein alpha-s internally tagged with mCherry and EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: G protein alpha-s-mCherry
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      336..345
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(548..567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(549..567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(612..663)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(641..661)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
       61 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatgctcc cgcccccacc
      121 ggatccctct ccgttaaacc cattaacatg tagaatcctc atctgcttca caatggtgct
      181 tttgccagac tctccagcac ccagcagcag caggcggtgc gtggcccggt agacctgctt
      241 gtccttctgc agctgcttct cgatcttttt gttggcctcg cgctgcgcct tctcctcgtt
      301 gcgctggtcc tcggtcttac tgttgccgag gcagcccatg gcggcggcgg ggcgcggccg
      361 ggctgcgggg cggcggggac ggggcgggcg gcctcgggcg ggccgggaaa gcggggaacg
      421 cggggagggt ggcggctcgg actaaggcaa gcgcgcggct ctccgcccct cgctccgggg
      481 ccgaggcgga gcagagcagg agccgcggga gctgctgctg cggaattggt cgaccaagct
      541 tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta
      601 gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      661 gtcaatgggg cggagttgtt acgacatttt ggaaa
//
