LOCUS       sequence-122200-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in 
            barley.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11057
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(61..136)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    627..651
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gcagaagaca atctgagcgt aatgccaact ttgtacaaga aagctgggtc tagaaaaaaa
       61 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      121 tttctagctc taaaacaaga gcccagagtg gagagcaagt ctgatgcagc aagcgagttt
      181 ataaaccggt cagaactctg ctggacaggc gaggtgggac taaactaaga ggaactgctg
      241 cgttgctctc cccaatcgtg aacacgtcga ccacccgaca aaagcccgat catgcgggcc
      301 taaaagccca gcgctagacc gttgctgaac tcacgtgggc tgcatctcac tatgcgaccc
      361 agatcagtga gagctagagg gcagttatct tcagctctct cgtagttcct ctgatcaata
      421 ttcctagttc gattccctcc tagcagtgag taacaatgtg ctccttgata aatataaatg
      481 tgttgagcac cagaactgtc agaataacgg gcttggtcct ccccatggga attcgtaatt
      541 gtcttcacag agtggggccc actgcatcca ccccagtaca ttaaaaacgt ccgcaatgtg
      601 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
      661 caacagctcc ccgaccggca gctcggcaca a
//
