LOCUS       sequence-122207-         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 
            in Brassica oleracea.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11062
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        229..304
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(316..340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    391..415
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gagcatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag
       61 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt
      121 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt
      181 atatacagct agagtcgaag tagtgattgt tgagagggga gccggtgagt tttagagcta
      241 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      301 gtgctttttt tctagaccca gctttcttgt acaaagttgg cattacgctc agattgtctt
      361 ctgcacgaag tggtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
      421 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
      481 tcgtccattt gtatgtgcat gccaaccaca gggttcccca gatcaggcgc tggctgctga
      541 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
      601 gaccca
//
