LOCUS       sequence-122212-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: Cas9 expression cassette for 
            monocotyledonous plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11056
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
       61 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
      121 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat
      181 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
      241 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
      301 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
      361 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
      421 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
      481 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtggagac
      541 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat
      601 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga
      661 ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac agccgccccc
      721 atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag agatgagcgt
      781 aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg gtgag
//
