LOCUS       sequence-122215-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 
            528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Mito-7
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..89
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..443
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    565..686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(602..621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     656..675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(830..851)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcagatccgc tagcatgtcc gtcctgacgc cgctgctgct gcggggcttg acaggctcgg
       61 cccggcggct cccagtgccg cgcgccaaga tccattcgtt gggggatcca ccggtcgcca
      121 ccatggagaa aagtttcgtg ataactgatc cacggctgcc agacaatccc atcatcttcg
      181 catccgatgg cttcctggag ctgaccgagt attccagaga ggagatcctg ggccgcaatg
      241 gccgctttct gcagggacca gagacagacc aggccacagt gcagaagatt cgcgatgcca
      301 ttagagatca gcgcgagatt accgtgcagc tgataaacta cacaaaaagc gggaagaaat
      361 tctggaacct cctgcacctc cagcccatga gggaccagaa gggtgagctc cagtatttca
      421 tcggagtgca gctggatgga taagcggccg cgactctaga tcataatcag ccataccaca
      481 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      541 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      661 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
      721 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
      781 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      841 caagagtcca ctattaaaga acgtggact
//
