LOCUS       sequence-122216-         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: Plant hygromycin (with intron) 
            resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11059
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          112..301
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
       61 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca ggtaaatttc
      121 tagtttttct ccttcatttt cttggttagg acccttttct ctttttattt ttttgagctt
      181 tgatctttct ttaaactgat ctatttttta attgattggt tatggtgtaa atattacata
      241 gctttaactg ataatctgat tactttattt cgtgtgtcta tgatgatgat gataactgca
      301 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
      361 cggcccattc ngaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
      421 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
      481 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aa
//
