LOCUS       sequence-122217-         366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: Cas9 expression cassette for plants 
            (exemplified in Brassica oleracea).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11060
FEATURES             Location/Qualifiers
     source          1..366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaaggtaatt atccaagatg tagcatcaag aatccaatgt ttacgggaaa aactatggaa
       61 gtattatgta agctcagcaa gaagcagatc aatatgcggc acatatgcaa cctatgttca
      121 aaaatgaaga atgtacagat acaagatcct atactgccag aatacgaaga agaatacgta
      181 gaaattgaaa aagaagaacc aggcgaagaa aagaatcttg atgacgtaag cactgacgac
      241 aacaatgaaa agaagaagat aaggtcggtg attgtgaaag agacatagag gacacatgta
      301 aggtggaaaa tgtaagggcg gaaagtaacc ttatcacaaa ggaatcttat cccccactac
      361 ttatcc
//
