LOCUS       sequence-122218-         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Golden Gate Cassette: Cas9 expression cassette for plants 
            (exemplified in Brassica oleracea).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL11060
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..47)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct
       61 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc
      121 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc
      181 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg
      241 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc
      301 cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat
      361 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg
      421 cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtggagact
      481 gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc
      541 atggatcaac tgcatgaagt tccggttggc aaatccatcg gacttaagaa aatccaggat
      601 tgtctttcca ctctgcttgt ctcggatccc attgatcagt tttcttgaca gccgccccca
      661 tcctgtatat cggcgcctct tgagctgttt catgactttg tcgtc
//
