LOCUS       sequence-122221-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Coding sequence, hygromycin 
            phophotransferase II (Escherichia coli).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL80036
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          372..561
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 tggccgattc attaatcact ctgtggtctc aaatgaaaaa gcctgaactc accgcgacgt
       61 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
      121 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
      181 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
      241 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
      301 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
      361 ctgttctgca ggtaaatttc tagtttttct ccttcatttt cttggttagg acccttttct
      421 ctttttattt ttttgagctt tgatctttct ttaaactgat ctatttttta attgattggt
      481 tatggtgtaa atattacata gctttaactg ataatctgat tactttattt cgtgtgtcta
      541 tgatgatgat gataactgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
      601 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
      661 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
      721 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
      781 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
      841 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttc
//
