LOCUS       sequence-122222-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Coding sequence, hygromycin 
            phophotransferase II (Escherichia coli).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL80036
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ataggcgtat cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcaaagccta
       61 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
      121 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
      181 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
      241 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
      301 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
      361 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
      421 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
      481 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
      541 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
      601 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
      661 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
      721 cgcatccatg gcctccgcga ccggctgcag ttatcatcat catcatagac acacgaaata
      781 aagtaatcag attatcagtt aaagctatgt aa
//
