LOCUS       sequence-122227-        1243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Coding sequence, neomycin 
            phophotransferase II (Escherichia coli).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL80037
FEATURES             Location/Qualifiers
     source          1..1243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..844
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(104..123)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     714..733
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(884..902)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acacgcctct ccgcgcgtng ccgattcatt aatcactctg tggtctcaaa tgattgaaca
       61 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
      121 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
      181 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
      241 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
      301 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
      361 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
      421 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
      481 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
      541 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
      601 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
      661 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
      721 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
      781 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
      841 ctgagctttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
      901 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      961 ggnaaatgtg cgcggaaccc cctatttgtt tattttttct aaatacattc aaatatgtat
     1021 ccgctcatga aacaataacc cntgataaat gcttcaataa tattgaaaaa aggaananta
     1081 tgcgctcncn caactggtcc aaaaccttga cnnanncanc ggtggtaacg ccncaatggc
     1141 ggtttncatg gcttgttaan acgntttttt ttggggtana ntctnngcct gggnnatcna
     1201 aaaanaaacc gtttaccccc tggntcaatt tttantttnn gaa
//
