LOCUS       sequence-122229-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL90003
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(572..589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(762..781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actcactata gggcgaattg agtgaaggcc gtcaaggcca cgtgtcttgt ccagagctcg
       61 gtctcaggag gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta
      121 tcaaggagca cattgttact cactgctagg agggaatcga actaggaata ttgatcagag
      181 gaactacgag agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt
      241 gagatgcagc ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat
      301 cgggcttttg tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc
      361 ttagtttagt cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc
      421 tgcatcagac ttgtgagacc ggtacctgga gcacaagact ggcctcatgg gccttcactt
      481 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct
      541 gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg
      601 ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa
      661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
//
